Degradation of hyaluronan by peroxynitrite

Min Li, Louis Rosenfeld, Rolondo E. Vilar, Mary K. Cowman

Research output: Contribution to journalArticlepeer-review

Abstract

Treatment of high-molecular-weight hyaluronan (HA) with peroxynitrite at neutral pH (ONOO-/ONOOH) results in altered mobility on agarose gel electrophoresis, as well as reduced limiting viscosity number. Both effects are consistent with a reduction in HA molecular weight. HA is protected from peroxynitrite attack to varying extents by addition of alternate target molecules. Thiourea is extremely effective as a protective agent, dimethyl sulfoxide is moderately effective, while sodium benzoate and mannitol are slightly effective. A similar pattern of protection is observed when HA is degraded by hydroxyl radical generated by a metal ion/hydrogen peroxide system. On the basis of these observations, peroxynitrite is proposed to have hydroxyl radical-like activity in degrading HA.

Original languageEnglish (US)
Pages (from-to)245-250
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume341
Issue number2
DOIs
StatePublished - May 15 1997

Keywords

  • hyaluronan
  • peroxynitrite

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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