Demonstration of retrotransposition of the Tf1 element in fission yeast

H. L. Levin, J. D. Boeke

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Tf1, a retrotransposon from fission yeast, has LTRs and coding sequences resembling the protease, reverse transcriptase and integrase domains of retroviral pol genes. A unique aspect of Tf1 is that it contains a single open reading frame whereas other retroviruses and retrotransposons usually possess two or more open reading frames. To determine whether Tf1 can transpose, we overproduced Tf1 transcripts encoded by a plasmid copy of the element marked with a neo gene. Approximately 0.1-4.0% of the cell population acquired chromosomally inherited resistance to G418. DNA blot analysis demonstrated that such strains had acquired both Tf1 and neo specific sequences within a restriction fragment of the same size; the size of this restriction fragment varied between different isolates. Structural analysis of the cloned DNA flanking the Tf1-neo element of two transposition candidates with the same regions in the parent strain showed that the ability to grow on G418 was due to transposition of Tf1-neo and not other types of recombination events.

    Original languageEnglish (US)
    Pages (from-to)1145-1153
    Number of pages9
    JournalEMBO Journal
    Volume11
    Issue number3
    DOIs
    StatePublished - 1992

    Keywords

    • Retrotransposition
    • Retrotransposon
    • Schizosaccharomyces pombe
    • Tf1
    • fbp promoter

    ASJC Scopus subject areas

    • Neuroscience(all)
    • Molecular Biology
    • Biochemistry, Genetics and Molecular Biology(all)
    • Immunology and Microbiology(all)

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