Dendrimers functionalized with membrane-interacting peptides for viral inhibition

Rossella Tarallo, Tom P. Carberry, Annarita Falanga, Mariateresa Vitiello, Stefania Galdiero, Massimiliano Galdiero, Marcus Weck

Research output: Contribution to journalArticle

Abstract

This contribution reports the synthesis of a poly(amide)-based dendrimer functionalized at the termini with a membrane-interacting peptide derived from the herpes simplex virus (HSV) type 1 glycoprotein H, namely gH625-644. This peptide has been shown to interact with model membranes and to inhibit viral infectivity. The peptidodendrimer inhibits both HSV-1 and HSV-2 at a very early stage of the entry process, most likely through an interaction with the viral envelope glycoproteins; thus, preventing the virus from coming into close contact with cellular membranes, a prerequisite of viral internalization. The 50% inhibitory concentration was 100 and 300 nM against HSV-1 and HSV-2 respectively, with no evidence of cell toxicity at these concentrations. These results show that the functionalization of a dendrimer with the peptide sequence derived from an HSV glycoprotein shows promising inhibitory activity towards viruses of the Herpesviridae family.

Original languageEnglish (US)
Pages (from-to)521-534
Number of pages14
JournalInternational Journal of Nanomedicine
Volume8
DOIs
StatePublished - Feb 2 2013

Keywords

  • Antiviral activity
  • Membranotropic peptides
  • Peptidodendrimer

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry

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