Desensitization of the neurokinin-1 receptor (NK1-R) in neurons: Effects of substance P on the distribution of NK1-R, G(αq/11), G-protein receptor kinase-2/3, and β-arrestin-1/2

Karen McConalogue, Carlos U. Corvera, Patrick D. Gamp, Eileen F. Grady, Nigel W. Bunnett

Research output: Contribution to journalArticlepeer-review

Abstract

Observations in reconstituted systems and transfected cells indicate that G-protein receptor kinases (GRKs) and β-arrestins mediate desensitization and endocytosis of G-protein-coupled receptors. Little is known about receptor regulation in neurons. Therefore, we examined the effects of the neurotransmitter substance P (SP) on desensitization of the neurokinin-1 receptor (NK1-R) and on the subcellular distribution of NK1-R, G(αq/11), GRK-2 and -3, and β-arrestin-1 and -2 in cultured myenteric neurons. NK1-R was coexpressed with immunoreactive G(αq/11), GRK-2 and -3, and β-arrestin-1 and -2 in a subpopulation of neurons. SP caused 1) rapid NK1-R-mediated increase in [Ca2+](i), which was transient and desensitized to repeated stimulation; 2) internalization of the NK1-R into early endosomes containing SP; and 3) rapid and transient redistribution of β-arrestin-1 and -2 from the cytosol to the plasma membrane, followed by a striking redistribution of β-arrestin-1 and -2 to endosomes containing the NK1-R and SP. In SP-treated neurons G(αq/11) remained at the plasma membrane, and GRK- 2 and -3 remained in centrally located and superficial vesicles. Thus, SP induces desensitization and endocytosis of the NK1-R in neurons that may be mediated by GRK-2 and -3 and β-arrestin-1 and -2. This regulation will determine whether NK1-R-expressing neurons participate in functionally important reflexes.

Original languageEnglish (US)
Pages (from-to)2305-2324
Number of pages20
JournalMolecular biology of the cell
Volume9
Issue number8
DOIs
StatePublished - Aug 1998

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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