Design-a-gene with genedesign

Sarah M. Richardson, Steffi Liu, Jef D. Boeke, Joel S. Bader

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Abstract

    The manual design of synthetic genes is a tedious and error-prone process-even for very short genes-and it becomes completely infeasible when multiple synthetic genes are needed. GeneDesign is a set of modules that automate batch nucleotide manipulation. Here, we explain the installation, configuration, and use of GeneDesign as part of a synthetic design workflow.

    Original languageEnglish (US)
    Title of host publicationGene Synthesis
    Subtitle of host publicationMethods and Protocols
    EditorsJean Peccoud
    Pages235-247
    Number of pages13
    DOIs
    StatePublished - 2012

    Publication series

    NameMethods in Molecular Biology
    Volume852
    ISSN (Print)1064-3745

    Keywords

    • Codon optimization
    • Computer-assisted GeneDesign
    • Synthetic biology
    • Synthetic biology software
    • Synthetic genes

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics

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