@inbook{f0c189af03004721849ad19d6b7a42b0,
title = "Design and characterization of fibers and bionanocomposites using the coiled-coil domain of cartilage oligomeric matrix protein",
abstract = "Tremendous effort has been dedicated to the design and assembly of bioinspired protein-based architectures with potential applications in drug delivery, tissue engineering, biosensing, and bioimaging. Here, we describe our strategy to generate fibers and bionanocomposites using the coiled-coil domain of cartilage oligomeric matrix protein (COMPcc). Our construct, Q, engineered by swapping particular regions of COMPcc to optimize surface charge, self-assembles to form nanofibers. The Q protein nanofibers can efficiently bind curcumin to form robust mesofibers that can be potentially used for drug delivery and biomedical applications. In addition, using the same Q protein, we describe the biotemplation of gold nanoparticles (AuNP) in the presence and absence of the hexahistidine tag (His-tag). The Q bearing His-tag·AuNP (Q·AuNP) readily deposits on electrode surfaces, while Q without His-tag·AuNP (Qx·AuNP) stabilizes the soluble protein·gold bionanocomposites for several days without aggregating.",
keywords = "Bionanocomposites, Cartilage oligomeric matrix protein, Coiled-coil protein, Domain-swapped, Fibers, Gold nanoparticles, Self-assembly",
author = "Priya Katyal and Montclare, {Jin Kim}",
note = "Funding Information: This work was supported by the National Science Foundation under award number NSFDMR-1728858. Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2018. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.",
year = "2018",
doi = "10.1007/978-1-4939-7893-9_19",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "239--263",
booktitle = "Methods in Molecular Biology",
}