Design and fabrication of a pdms microfluidic probe and perfusion chamber for microfluidic experiments with organotypic brain slices

Arthur Queval, Cécile M. Perrault, Mohammad A. Qasaimeh, R. A. McKinney, David Juncker

Research output: Contribution to conferencePaperpeer-review

Abstract

Microfluidic systems are increasingly being used for the culture and study of dissociated cells because they facilitate drug screening and chemotaxis studies using minute amounts of chemicals. However, microfluidics have not been adopted for brain slices because of the challenges associated with the culture conditions necessary for maintaining the integrity of the slices. Here, we present a microfluidic system comprising a perfusion chamber compatible with confocal microscopy and a transparent microfluidic probe (MFP) for local microperfusion of organotypic brain slices for epilepsy studies.

Original languageEnglish (US)
Pages1663-1665
Number of pages3
StatePublished - 2008
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: Oct 12 2008Oct 16 2008

Other

Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period10/12/0810/16/08

Keywords

  • Microfluidic probe
  • Organotypic slices
  • Superfusion

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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