Design and fabrication of a pdms microfluidic probe and perfusion chamber for microfluidic experiments with organotypic brain slices

Arthur Queval; Cécile M. Perrault; Mohammad A. Qasaimeh; R. A. McKinney; David Juncker

Design and fabrication of a pdms microfluidic probe and perfusion chamber for microfluidic experiments with organotypic brain slices

Arthur Queval, Cécile M. Perrault, Mohammad A. Qasaimeh, R. A. McKinney, David Juncker

Mechanical Engineering

Research output: Contribution to conference › Paper › peer-review

Abstract

Microfluidic systems are increasingly being used for the culture and study of dissociated cells because they facilitate drug screening and chemotaxis studies using minute amounts of chemicals. However, microfluidics have not been adopted for brain slices because of the challenges associated with the culture conditions necessary for maintaining the integrity of the slices. Here, we present a microfluidic system comprising a perfusion chamber compatible with confocal microscopy and a transparent microfluidic probe (MFP) for local microperfusion of organotypic brain slices for epilepsy studies.

Original language	English (US)
Pages	1663-1665
Number of pages	3
State	Published - 2008
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: Oct 12 2008 → Oct 16 2008

Other

Other	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	10/12/08 → 10/16/08

Keywords

Microfluidic probe
Organotypic slices
Superfusion

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

Queval, A., Perrault, C. M., Qasaimeh, M. A., McKinney, R. A., & Juncker, D. (2008). Design and fabrication of a pdms microfluidic probe and perfusion chamber for microfluidic experiments with organotypic brain slices. 1663-1665. Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.

Design and fabrication of a pdms microfluidic probe and perfusion chamber for microfluidic experiments with organotypic brain slices. / Queval, Arthur; Perrault, Cécile M.; Qasaimeh, Mohammad A. et al.
2008. 1663-1665 Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.

Research output: Contribution to conference › Paper › peer-review

Queval, A, Perrault, CM, Qasaimeh, MA, McKinney, RA & Juncker, D 2008, 'Design and fabrication of a pdms microfluidic probe and perfusion chamber for microfluidic experiments with organotypic brain slices', Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States, 10/12/08 - 10/16/08 pp. 1663-1665.

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AU - Queval, Arthur

AU - Perrault, Cécile M.

AU - Qasaimeh, Mohammad A.

AU - McKinney, R. A.

AU - Juncker, David

PY - 2008

Y1 - 2008

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AB - Microfluidic systems are increasingly being used for the culture and study of dissociated cells because they facilitate drug screening and chemotaxis studies using minute amounts of chemicals. However, microfluidics have not been adopted for brain slices because of the challenges associated with the culture conditions necessary for maintaining the integrity of the slices. Here, we present a microfluidic system comprising a perfusion chamber compatible with confocal microscopy and a transparent microfluidic probe (MFP) for local microperfusion of organotypic brain slices for epilepsy studies.

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KW - Organotypic slices

KW - Superfusion

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Y2 - 12 October 2008 through 16 October 2008

ER -

Design and fabrication of a pdms microfluidic probe and perfusion chamber for microfluidic experiments with organotypic brain slices

Abstract

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Keywords

ASJC Scopus subject areas

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