Design of a synthetic yeast genome

Sarah M. Richardson, Leslie A. Mitchell, Giovanni Stracquadanio, Kun Yang, Jessica S. Dymond, James E. DiCarlo, Dongwon Lee, Cheng Lai Victor Huang, Srinivasan Chandrasegaran, Yizhi Cai, Jef D. Boeke, Joel S. Bader

    Research output: Contribution to journalArticle

    Abstract

    We describe complete design of a synthetic eukaryotic genome, Sc2.0, a highly modified Saccharomyces cerevisiae genome reduced in size by nearly 8%, with 1.1 megabases of the synthetic genome deleted, inserted, or altered. Sc2.0 chromosome design was implemented with BioStudio, an open-source framework developed for eukaryotic genome design, which coordinates design modifications from nucleotide to genome scales and enforces version control to systematically track edits. To achieve complete Sc2.0 genome synthesis, individual synthetic chromosomes built by Sc2.0 Consortium teams around the world will be consolidated into a single strain by "endoreduplication intercross." Chemically synthesized genomes like Sc2.0 are fully customizable and allow experimentalists to ask otherwise intractable questions about chromosome structure, function, and evolution with a bottom-up design strategy.

    Original languageEnglish (US)
    Pages (from-to)1040-1044
    Number of pages5
    JournalScience
    Volume355
    Issue number6329
    DOIs
    StatePublished - Mar 10 2017

    ASJC Scopus subject areas

    • General

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  • Cite this

    Richardson, S. M., Mitchell, L. A., Stracquadanio, G., Yang, K., Dymond, J. S., DiCarlo, J. E., Lee, D., Huang, C. L. V., Chandrasegaran, S., Cai, Y., Boeke, J. D., & Bader, J. S. (2017). Design of a synthetic yeast genome. Science, 355(6329), 1040-1044. https://doi.org/10.1126/science.aaf4557