@article{9fbfe3ee0f904c46921505397a59b24b,
title = "Designing Higher Resolution Self-Assembled 3D DNA Crystals via Strand Terminus Modifications",
abstract = "DNA tensegrity triangles self-assemble into rhombohedral three-dimensional crystals via sticky ended cohesion. Crystals containing two-nucleotide (nt) sticky ends (GA:TC) have been reported previously, and those crystals diffracted to 4.9 {\AA} at beamline NSLS-I-X25. Here, we analyze the effect of varying sticky end lengths and sequences as well as the impact of 5′- and 3′-phosphates on crystal formation and resolution. Tensegrity triangle motifs having 1-, 2-, 3-, and 4-nt sticky ends all form crystals. X-ray diffraction data from the same beamline reveal that the crystal resolution for a 1-nt sticky end (G:C) and a 3-nt sticky end (GAT:ATC) were 3.4 and 4.2 {\AA}, respectively. Resolutions were determined from complete data sets in each case. We also conducted trials that examined every possible combination of 1-nucleotide and 2-nucleotide sticky-ended phosphorylated strands and successfully crystallized all 16 possible combinations of strands. We observed the position of the 5′-phosphate on either the crossover (1), helical (2), or central strand (3) affected the resolution of the self-assembled crystals for the 2-turn monomer (3.0 {\AA} for 1-2P-3P) and 2-turn dimer sticky ended (4.1 {\AA} for 1-2-3P) systems. We have also examined the impact of the identity of the base flanking the sticky ends as well as the use of 3′-phosphate. We conclude that crystal resolution is not a simple consequence of the thermodynamics of the direct nucleotide pairing interactions involved in molecular cohesion in this system.",
keywords = "Crystalline order optimization, DNA crystals, Self-assembly, Sticky ends, Terminal phosphates",
author = "Ohayon, {Yoel P.} and Carina Hernandez and Chandrasekaran, {Arun Richard} and Xinyu Wang and Abdallah, {Hatem O.} and Jong, {Michael Alexander} and Mohsen, {Michael G.} and Ruojie Sha and Birktoft, {Jens J.} and Lukeman, {Philip S.} and Chaikin, {Paul M.} and Ginell, {Stephen L.} and Chengde Mao and Seeman, {Nadrian C.}",
note = "Funding Information: We acknowledge support of the following grants to N.C.S.: Grant No. GM-29554 from NIGMS, Grant Nos. CTS-1120890, CCF-1117210, EFRI-1332411, and CHE-1708776 from the NSF, Grant No. W911NF-1110024 from ARO, Grant Nos. N000141110729 and N000140911118 from ONR, DE-SC0007991 from DOE for DNA synthesis, and partial salary support and GBMF3849 from the Gordon and Betty Moore Foundation. Use of the National Synchrotron Light Source, Brookhaven National Laboratory, was supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, under Contract No. DE-AC02-98CH10886. Funding Information: We acknowledge support of the following grants to N.C.S.: Grant No. GM-29554 from NIGMS, Grant Nos. CTS-1120890, CCF-1117210 EFRI-1332411, and CHE-1708776 from the NSF, Grant No. W911NF-1110024 from ARO, Grant Nos. N000141110729 and N000140911118 from ONR, DE-SC0007991 from DOE for DNA synthesis, and partial salary support and GBMF3849 from the Gordon and Betty Moore Foundation. Use of the National Synchrotron Light Source, Brookhaven National Laboratory, was supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, under Contract No. DE-AC02-98CH10886. We acknowledge support of the following grants to N.C.S.: Grant No. GM-29554 from NIGMS, Grant Nos. CTS-1120890, CCF-1117210 EFRI-1332411, and CHE-1708776 from the NSF, Grant No. W911NF-1110024 from ARO, Grant Nos. N000141110729 and N000140911118 from ONR, DE-SC0007991 from DOE for DNA synthesis, and partial salary support and GBMF3849 from the Gordon and Betty Moore Foundation. Use of the National Synchrotron Light Source, Brookhaven National Laboratory, was supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, under Contract No. DE-AC02-98CH10886 Publisher Copyright: {\textcopyright} Copyright 2019 American Chemical Society.",
year = "2019",
month = jul,
day = "23",
doi = "10.1021/acsnano.9b02430",
language = "English (US)",
volume = "13",
pages = "7957--7965",
journal = "ACS Nano",
issn = "1936-0851",
publisher = "American Chemical Society",
number = "7",
}