Designing protein denaturants: Synthetic agents induce cytochrome c unfolding at low concentrations and stoichiometries

Rishi K. Jain, Andrew D. Hamilton

Research output: Contribution to journalArticle

Abstract

The structural diversity and marginal stability (5-15 kcal mol-1) of proteins suggest the development of synthetic agents that selectively promote denaturation at low concentrations (see scheme). It is shown that an artificial receptor that binds tightly to the surface of native cytochrome c (cyt. c) lowers the melting temperature of the protein by 20°C at stoichiometric amounts; the receptor also selectively binds the native from rather than cyt. C551 and surface-modified cyt. c.

Original languageEnglish (US)
Pages (from-to)641-643
Number of pages3
JournalAngewandte Chemie - International Edition
Volume41
Issue number4
DOIs
StatePublished - Feb 15 2002

Keywords

  • Denaturation
  • Molecular recognition
  • Porphyrinoids
  • Protein folding
  • Protein surface

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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