Designing protein denaturants: Synthetic agents induce cytochrome c unfolding at low concentrations and stoichiometries

Rishi K. Jain; Andrew D. Hamilton

doi:10.1002/1521-3773(20020215)41:4<641::AID-ANIE641>3.0.CO;2-1

Designing protein denaturants: Synthetic agents induce cytochrome c unfolding at low concentrations and stoichiometries

Rishi K. Jain, Andrew D. Hamilton

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

The structural diversity and marginal stability (5-15 kcal mol^-1) of proteins suggest the development of synthetic agents that selectively promote denaturation at low concentrations (see scheme). It is shown that an artificial receptor that binds tightly to the surface of native cytochrome c (cyt. c) lowers the melting temperature of the protein by 20°C at stoichiometric amounts; the receptor also selectively binds the native from rather than cyt. C551 and surface-modified cyt. c.

Original language	English (US)
Pages (from-to)	641-643
Number of pages	3
Journal	Angewandte Chemie - International Edition
Volume	41
Issue number	4
DOIs	https://doi.org/10.1002/1521-3773(20020215)41:4<641::AID-ANIE641>3.0.CO;2-1
State	Published - Feb 15 2002

Keywords

Denaturation
Molecular recognition
Porphyrinoids
Protein folding
Protein surface

ASJC Scopus subject areas

Catalysis
General Chemistry

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10.1002/1521-3773(20020215)41:4<641::AID-ANIE641>3.0.CO;2-1

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Designing protein denaturants: Synthetic agents induce cytochrome c unfolding at low concentrations and stoichiometries

Abstract

Keywords

ASJC Scopus subject areas

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