Detecting gene copy number fluctuations in tumor cells by microarray analysis of genomic representations

Robert Lucito, Joseph West, Andrew Reiner, Joan Alexander, Diane Esposito, Bhubaneswar Mishra, Scott Powers, Larry Norton, Michael Wigler

Research output: Contribution to journalArticle

Abstract

In this work, we explore the use of representations in conjunction with DNA microarray technology to measure gene copy number changes in cancer. We demonstrate that arrays of DNA probes derived from low-complexity representations can be used to detect amplifications, deletions, and polymorphic differences when hybridized to representations of genomic DNA. The method is both reproducible and verifiable, and is applicable even to microscopic amounts of primary tumors. We also present a mathematical model for array performance that is useful for designing and understanding DNA microarray hybridization protocols. The future applications and challenges of this approach are discussed.

Original languageEnglish (US)
Pages (from-to)1726-1736
Number of pages11
JournalGenome Research
Volume10
Issue number11
DOIs
StatePublished - Dec 21 2000

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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  • Cite this

    Lucito, R., West, J., Reiner, A., Alexander, J., Esposito, D., Mishra, B., Powers, S., Norton, L., & Wigler, M. (2000). Detecting gene copy number fluctuations in tumor cells by microarray analysis of genomic representations. Genome Research, 10(11), 1726-1736. https://doi.org/10.1101/gr.138300