Detecting single stranded DNA with a solid state nanopore

Daniel Fologea; Marc Gershow; Bradley Ledden; David S. McNabb; Jene A. Golovchenko; Jiali Li

doi:10.1021/nl051199m

Detecting single stranded DNA with a solid state nanopore

Daniel Fologea, Marc Gershow, Bradley Ledden, David S. McNabb, Jene A. Golovchenko, Jiali Li

Research output: Contribution to journal › Article › peer-review

Abstract

Voltage biased solid-state nanopores are used to detect and characterize individual single stranded DNA molecules of fixed micrometer length by operating a nanopore detector at pH values greater than ∼11.6. The distribution of observed molecular event durations and blockade currents shows that a significant fraction of the events obey a rule of constant event charge deficit (ecd) indicating that they correspond to molecules translocating through the nanopore in a distribution of folded and unfolded configurations. A surprisingly large component is unfolded. The result is an important milestone in developing solid-state nanopores for single molecule sequencing applications.

Original language	English (US)
Pages (from-to)	1905-1909
Number of pages	5
Journal	Nano Letters
Volume	5
Issue number	10
DOIs	https://doi.org/10.1021/nl051199m
State	Published - Oct 2005

ASJC Scopus subject areas

Bioengineering
General Chemistry
General Materials Science
Condensed Matter Physics
Mechanical Engineering

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10.1021/nl051199m

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AU - Ledden, Bradley

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AU - Golovchenko, Jene A.

AU - Li, Jiali

PY - 2005/10

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AB - Voltage biased solid-state nanopores are used to detect and characterize individual single stranded DNA molecules of fixed micrometer length by operating a nanopore detector at pH values greater than ∼11.6. The distribution of observed molecular event durations and blockade currents shows that a significant fraction of the events obey a rule of constant event charge deficit (ecd) indicating that they correspond to molecules translocating through the nanopore in a distribution of folded and unfolded configurations. A surprisingly large component is unfolded. The result is an important milestone in developing solid-state nanopores for single molecule sequencing applications.

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Detecting single stranded DNA with a solid state nanopore

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