Detecting single stranded DNA with a solid state nanopore

Daniel Fologea, Marc Gershow, Bradley Ledden, David S. McNabb, Jene A. Golovchenko, Jiali Li

    Research output: Contribution to journalArticle

    Abstract

    Voltage biased solid-state nanopores are used to detect and characterize individual single stranded DNA molecules of fixed micrometer length by operating a nanopore detector at pH values greater than ∼11.6. The distribution of observed molecular event durations and blockade currents shows that a significant fraction of the events obey a rule of constant event charge deficit (ecd) indicating that they correspond to molecules translocating through the nanopore in a distribution of folded and unfolded configurations. A surprisingly large component is unfolded. The result is an important milestone in developing solid-state nanopores for single molecule sequencing applications.

    Original languageEnglish (US)
    Pages (from-to)1905-1909
    Number of pages5
    JournalNano Letters
    Volume5
    Issue number10
    DOIs
    StatePublished - Oct 1 2005

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    ASJC Scopus subject areas

    • Bioengineering
    • Chemistry(all)
    • Materials Science(all)
    • Condensed Matter Physics
    • Mechanical Engineering

    Cite this

    Fologea, D., Gershow, M., Ledden, B., McNabb, D. S., Golovchenko, J. A., & Li, J. (2005). Detecting single stranded DNA with a solid state nanopore. Nano Letters, 5(10), 1905-1909. https://doi.org/10.1021/nl051199m