Detecting single stranded DNA with a solid state nanopore

Daniel Fologea, Marc Gershow, Bradley Ledden, David S. McNabb, Jene A. Golovchenko, Jiali Li

    Research output: Contribution to journalArticlepeer-review


    Voltage biased solid-state nanopores are used to detect and characterize individual single stranded DNA molecules of fixed micrometer length by operating a nanopore detector at pH values greater than ∼11.6. The distribution of observed molecular event durations and blockade currents shows that a significant fraction of the events obey a rule of constant event charge deficit (ecd) indicating that they correspond to molecules translocating through the nanopore in a distribution of folded and unfolded configurations. A surprisingly large component is unfolded. The result is an important milestone in developing solid-state nanopores for single molecule sequencing applications.

    Original languageEnglish (US)
    Pages (from-to)1905-1909
    Number of pages5
    JournalNano Letters
    Issue number10
    StatePublished - Oct 2005

    ASJC Scopus subject areas

    • Bioengineering
    • General Chemistry
    • General Materials Science
    • Condensed Matter Physics
    • Mechanical Engineering


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