Detection of HHV-6B in post-mortem central nervous system tissue of a post-bone marrow transplant recipient: a multi-virus array analysis.

Karen Yao; Nahid Akyani; Donatella Donati; Naomi Sengamalay; Julie Fotheringham; Elodie Ghedin; Michael Bishop; John Barrett; Fatah Kashanchi; Steven Jacobson

doi:10.1016/S1386-6532(06)70013-0

Detection of HHV-6B in post-mortem central nervous system tissue of a post-bone marrow transplant recipient: a multi-virus array analysis.

Karen Yao, Nahid Akyani, Donatella Donati, Naomi Sengamalay, Julie Fotheringham, Elodie Ghedin, Michael Bishop, John Barrett, Fatah Kashanchi, Steven Jacobson

Research output: Contribution to journal › Article › peer-review

Abstract

BACKGROUND: HHV-6 has been implicated in a number of neurological disorders. Recent evidence has suggested high incidence of HHV-6 infection in patients (46%) undergoing allogeneic bone marrow transplant (BMT). OBJECTIVE: To investigate whether HHV-6 plays a role in the development of fatal encephalopathy in an allogeneic post-BMT patient using an unbiased approach. RESULTS: Detection of HHV-6 viral DNA sequence and RNA expression were demonstrated in fresh frozen post-mortem autopsy material derived from the insular cortex using a multi-virus array platform. In addition, PCR analysis by real-time quantitative TaqMan demonstrated high viral burden in multiple brain regions tested. Sequencing analysis of PCR product confirmed the virus to be HHV-6 variant B. CONCLUSIONS: Active infection as demonstrated by expression of viral RNA and high viral load in the CNS suggest a possible pathogenic role of HHV-6 in development neurologic complications post-BMT.

Original language	English (US)
Pages (from-to)	S57-62
Journal	Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
Volume	37 Suppl 1
DOIs	https://doi.org/10.1016/S1386-6532(06)70013-0
State	Published - Dec 2006

ASJC Scopus subject areas

Virology
Infectious Diseases

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10.1016/S1386-6532(06)70013-0

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Yao, K., Akyani, N., Donati, D., Sengamalay, N., Fotheringham, J., Ghedin, E., Bishop, M., Barrett, J., Kashanchi, F., & Jacobson, S. (2006). Detection of HHV-6B in post-mortem central nervous system tissue of a post-bone marrow transplant recipient: a multi-virus array analysis. Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 37 Suppl 1, S57-62. https://doi.org/10.1016/S1386-6532(06)70013-0

Detection of HHV-6B in post-mortem central nervous system tissue of a post-bone marrow transplant recipient: a multi-virus array analysis. / Yao, Karen; Akyani, Nahid; Donati, Donatella et al.
In: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, Vol. 37 Suppl 1, 12.2006, p. S57-62.

Research output: Contribution to journal › Article › peer-review

Yao, K, Akyani, N, Donati, D, Sengamalay, N, Fotheringham, J, Ghedin, E, Bishop, M, Barrett, J, Kashanchi, F & Jacobson, S 2006, 'Detection of HHV-6B in post-mortem central nervous system tissue of a post-bone marrow transplant recipient: a multi-virus array analysis.', Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, vol. 37 Suppl 1, pp. S57-62. https://doi.org/10.1016/S1386-6532(06)70013-0

Yao K, Akyani N, Donati D, Sengamalay N, Fotheringham J, Ghedin E et al. Detection of HHV-6B in post-mortem central nervous system tissue of a post-bone marrow transplant recipient: a multi-virus array analysis. Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology. 2006 Dec;37 Suppl 1:S57-62. doi: 10.1016/S1386-6532(06)70013-0

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title = "Detection of HHV-6B in post-mortem central nervous system tissue of a post-bone marrow transplant recipient: a multi-virus array analysis.",

abstract = "BACKGROUND: HHV-6 has been implicated in a number of neurological disorders. Recent evidence has suggested high incidence of HHV-6 infection in patients (46%) undergoing allogeneic bone marrow transplant (BMT). OBJECTIVE: To investigate whether HHV-6 plays a role in the development of fatal encephalopathy in an allogeneic post-BMT patient using an unbiased approach. RESULTS: Detection of HHV-6 viral DNA sequence and RNA expression were demonstrated in fresh frozen post-mortem autopsy material derived from the insular cortex using a multi-virus array platform. In addition, PCR analysis by real-time quantitative TaqMan demonstrated high viral burden in multiple brain regions tested. Sequencing analysis of PCR product confirmed the virus to be HHV-6 variant B. CONCLUSIONS: Active infection as demonstrated by expression of viral RNA and high viral load in the CNS suggest a possible pathogenic role of HHV-6 in development neurologic complications post-BMT.",

author = "Karen Yao and Nahid Akyani and Donatella Donati and Naomi Sengamalay and Julie Fotheringham and Elodie Ghedin and Michael Bishop and John Barrett and Fatah Kashanchi and Steven Jacobson",

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doi = "10.1016/S1386-6532(06)70013-0",

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AU - Yao, Karen

AU - Akyani, Nahid

AU - Donati, Donatella

AU - Sengamalay, Naomi

AU - Fotheringham, Julie

AU - Ghedin, Elodie

AU - Bishop, Michael

AU - Barrett, John

AU - Kashanchi, Fatah

AU - Jacobson, Steven

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N2 - BACKGROUND: HHV-6 has been implicated in a number of neurological disorders. Recent evidence has suggested high incidence of HHV-6 infection in patients (46%) undergoing allogeneic bone marrow transplant (BMT). OBJECTIVE: To investigate whether HHV-6 plays a role in the development of fatal encephalopathy in an allogeneic post-BMT patient using an unbiased approach. RESULTS: Detection of HHV-6 viral DNA sequence and RNA expression were demonstrated in fresh frozen post-mortem autopsy material derived from the insular cortex using a multi-virus array platform. In addition, PCR analysis by real-time quantitative TaqMan demonstrated high viral burden in multiple brain regions tested. Sequencing analysis of PCR product confirmed the virus to be HHV-6 variant B. CONCLUSIONS: Active infection as demonstrated by expression of viral RNA and high viral load in the CNS suggest a possible pathogenic role of HHV-6 in development neurologic complications post-BMT.

AB - BACKGROUND: HHV-6 has been implicated in a number of neurological disorders. Recent evidence has suggested high incidence of HHV-6 infection in patients (46%) undergoing allogeneic bone marrow transplant (BMT). OBJECTIVE: To investigate whether HHV-6 plays a role in the development of fatal encephalopathy in an allogeneic post-BMT patient using an unbiased approach. RESULTS: Detection of HHV-6 viral DNA sequence and RNA expression were demonstrated in fresh frozen post-mortem autopsy material derived from the insular cortex using a multi-virus array platform. In addition, PCR analysis by real-time quantitative TaqMan demonstrated high viral burden in multiple brain regions tested. Sequencing analysis of PCR product confirmed the virus to be HHV-6 variant B. CONCLUSIONS: Active infection as demonstrated by expression of viral RNA and high viral load in the CNS suggest a possible pathogenic role of HHV-6 in development neurologic complications post-BMT.

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Detection of HHV-6B in post-mortem central nervous system tissue of a post-bone marrow transplant recipient: a multi-virus array analysis.

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