TY - JOUR
T1 - Detection of HHV-6B in post-mortem central nervous system tissue of a post-bone marrow transplant recipient
T2 - a multi-virus array analysis.
AU - Yao, Karen
AU - Akyani, Nahid
AU - Donati, Donatella
AU - Sengamalay, Naomi
AU - Fotheringham, Julie
AU - Ghedin, Elodie
AU - Bishop, Michael
AU - Barrett, John
AU - Kashanchi, Fatah
AU - Jacobson, Steven
PY - 2006/12
Y1 - 2006/12
N2 - BACKGROUND: HHV-6 has been implicated in a number of neurological disorders. Recent evidence has suggested high incidence of HHV-6 infection in patients (46%) undergoing allogeneic bone marrow transplant (BMT). OBJECTIVE: To investigate whether HHV-6 plays a role in the development of fatal encephalopathy in an allogeneic post-BMT patient using an unbiased approach. RESULTS: Detection of HHV-6 viral DNA sequence and RNA expression were demonstrated in fresh frozen post-mortem autopsy material derived from the insular cortex using a multi-virus array platform. In addition, PCR analysis by real-time quantitative TaqMan demonstrated high viral burden in multiple brain regions tested. Sequencing analysis of PCR product confirmed the virus to be HHV-6 variant B. CONCLUSIONS: Active infection as demonstrated by expression of viral RNA and high viral load in the CNS suggest a possible pathogenic role of HHV-6 in development neurologic complications post-BMT.
AB - BACKGROUND: HHV-6 has been implicated in a number of neurological disorders. Recent evidence has suggested high incidence of HHV-6 infection in patients (46%) undergoing allogeneic bone marrow transplant (BMT). OBJECTIVE: To investigate whether HHV-6 plays a role in the development of fatal encephalopathy in an allogeneic post-BMT patient using an unbiased approach. RESULTS: Detection of HHV-6 viral DNA sequence and RNA expression were demonstrated in fresh frozen post-mortem autopsy material derived from the insular cortex using a multi-virus array platform. In addition, PCR analysis by real-time quantitative TaqMan demonstrated high viral burden in multiple brain regions tested. Sequencing analysis of PCR product confirmed the virus to be HHV-6 variant B. CONCLUSIONS: Active infection as demonstrated by expression of viral RNA and high viral load in the CNS suggest a possible pathogenic role of HHV-6 in development neurologic complications post-BMT.
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U2 - 10.1016/S1386-6532(06)70013-0
DO - 10.1016/S1386-6532(06)70013-0
M3 - Article
C2 - 17276371
AN - SCOPUS:34548047667
VL - 37 Suppl 1
SP - S57-62
JO - Journal of Clinical Virology
JF - Journal of Clinical Virology
SN - 1386-6532
ER -