Detection of nitric oxide and nitroxyl with benzoresorufin-based fluorescent sensors

Ulf Peter Apfel; Daniela Buccella; Justin J. Wilson; Stephen J. Lippard

doi:10.1021/ic302793w

Detection of nitric oxide and nitroxyl with benzoresorufin-based fluorescent sensors

Ulf Peter Apfel, Daniela Buccella, Justin J. Wilson, Stephen J. Lippard

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

A new family of benzoresorufin-based copper complexes for fluorescence detection of NO and HNO is reported. The copper complexes, CuBRNO1-3, elicit 1.5-4.8-fold emission enhancement in response to NO and HNO. The three sensors differ in the nature of the metal-binding site. The photophysical properties of these sensors are investigated with assistance from density functional theory calculations. The fluorescence turn-on observed upon reaction with HNO is an unexpected result that is discussed in detail. The utility of the new sensors for detecting HNO and NO in HeLa cells and RAW 264.7 macrophages is demonstrated.

Original language	English (US)
Pages (from-to)	3285-3294
Number of pages	10
Journal	Inorganic Chemistry
Volume	52
Issue number	6
DOIs	https://doi.org/10.1021/ic302793w
State	Published - Mar 18 2013

ASJC Scopus subject areas

Physical and Theoretical Chemistry
Inorganic Chemistry

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10.1021/ic302793w

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title = "Detection of nitric oxide and nitroxyl with benzoresorufin-based fluorescent sensors",

abstract = "A new family of benzoresorufin-based copper complexes for fluorescence detection of NO and HNO is reported. The copper complexes, CuBRNO1-3, elicit 1.5-4.8-fold emission enhancement in response to NO and HNO. The three sensors differ in the nature of the metal-binding site. The photophysical properties of these sensors are investigated with assistance from density functional theory calculations. The fluorescence turn-on observed upon reaction with HNO is an unexpected result that is discussed in detail. The utility of the new sensors for detecting HNO and NO in HeLa cells and RAW 264.7 macrophages is demonstrated.",

author = "Apfel, {Ulf Peter} and Daniela Buccella and Wilson, {Justin J.} and Lippard, {Stephen J.}",

year = "2013",

month = mar,

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doi = "10.1021/ic302793w",

language = "English (US)",

volume = "52",

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journal = "Inorganic Chemistry",

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AU - Apfel, Ulf Peter

AU - Buccella, Daniela

AU - Wilson, Justin J.

AU - Lippard, Stephen J.

PY - 2013/3/18

Y1 - 2013/3/18

N2 - A new family of benzoresorufin-based copper complexes for fluorescence detection of NO and HNO is reported. The copper complexes, CuBRNO1-3, elicit 1.5-4.8-fold emission enhancement in response to NO and HNO. The three sensors differ in the nature of the metal-binding site. The photophysical properties of these sensors are investigated with assistance from density functional theory calculations. The fluorescence turn-on observed upon reaction with HNO is an unexpected result that is discussed in detail. The utility of the new sensors for detecting HNO and NO in HeLa cells and RAW 264.7 macrophages is demonstrated.

AB - A new family of benzoresorufin-based copper complexes for fluorescence detection of NO and HNO is reported. The copper complexes, CuBRNO1-3, elicit 1.5-4.8-fold emission enhancement in response to NO and HNO. The three sensors differ in the nature of the metal-binding site. The photophysical properties of these sensors are investigated with assistance from density functional theory calculations. The fluorescence turn-on observed upon reaction with HNO is an unexpected result that is discussed in detail. The utility of the new sensors for detecting HNO and NO in HeLa cells and RAW 264.7 macrophages is demonstrated.

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JO - Inorganic Chemistry

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Detection of nitric oxide and nitroxyl with benzoresorufin-based fluorescent sensors

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