Detection of nitric oxide and nitroxyl with benzoresorufin-based fluorescent sensors

Ulf Peter Apfel, Daniela Buccella, Justin J. Wilson, Stephen J. Lippard

Research output: Contribution to journalArticlepeer-review


A new family of benzoresorufin-based copper complexes for fluorescence detection of NO and HNO is reported. The copper complexes, CuBRNO1-3, elicit 1.5-4.8-fold emission enhancement in response to NO and HNO. The three sensors differ in the nature of the metal-binding site. The photophysical properties of these sensors are investigated with assistance from density functional theory calculations. The fluorescence turn-on observed upon reaction with HNO is an unexpected result that is discussed in detail. The utility of the new sensors for detecting HNO and NO in HeLa cells and RAW 264.7 macrophages is demonstrated.

Original languageEnglish (US)
Pages (from-to)3285-3294
Number of pages10
JournalInorganic Chemistry
Issue number6
StatePublished - Mar 18 2013

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Inorganic Chemistry


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