Detection of single molecules in microspheres

Michael D. Barnes, William B. Whiuen, J. Michael Ramsey, Kin C. Ng, Stephen Arnold

Research output: Contribution to journalConference articlepeer-review


We have investigated the use of micron-sized liquid droplets as sample medium to detect single fluorescent molecules in solution. The use of microdroplets (5-15 jim diameter) offers several powerful advantages over single-molecule detection schemes involving measurements on bulk liquids where the probe volume is defined by the laser beam. In addition, cavity-quantum electrodynamical (QED) effects have been observed which influence both spontaneous emission rates and fluorescence yields of dye molecules in these microspheres.

Original languageEnglish (US)
Pages (from-to)458-465
Number of pages8
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - May 18 1993
EventAdvances in Fluorescence Sensing Technology 1993 - Los Angeles, United States
Duration: Jan 17 1993Jan 22 1993

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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