Detection of single molecules in microspheres

Michael D. Barnes; William B. Whiuen; J. Michael Ramsey; Kin C. Ng; Stephen Arnold

doi:10.1117/12.144732

Detection of single molecules in microspheres

Michael D. Barnes, William B. Whiuen, J. Michael Ramsey, Kin C. Ng, Stephen Arnold

Research output: Contribution to journal › Conference article › peer-review

Abstract

We have investigated the use of micron-sized liquid droplets as sample medium to detect single fluorescent molecules in solution. The use of microdroplets (5-15 jim diameter) offers several powerful advantages over single-molecule detection schemes involving measurements on bulk liquids where the probe volume is defined by the laser beam. In addition, cavity-quantum electrodynamical (QED) effects have been observed which influence both spontaneous emission rates and fluorescence yields of dye molecules in these microspheres.

Original language	English (US)
Pages (from-to)	458-465
Number of pages	8
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	1885
DOIs	https://doi.org/10.1117/12.144732
State	Published - May 18 1993
Event	Advances in Fluorescence Sensing Technology 1993 - Los Angeles, United States Duration: Jan 17 1993 → Jan 22 1993

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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10.1117/12.144732

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title = "Detection of single molecules in microspheres",

abstract = "We have investigated the use of micron-sized liquid droplets as sample medium to detect single fluorescent molecules in solution. The use of microdroplets (5-15 jim diameter) offers several powerful advantages over single-molecule detection schemes involving measurements on bulk liquids where the probe volume is defined by the laser beam. In addition, cavity-quantum electrodynamical (QED) effects have been observed which influence both spontaneous emission rates and fluorescence yields of dye molecules in these microspheres.",

author = "Barnes, {Michael D.} and Whiuen, {William B.} and Ramsey, {J. Michael} and Ng, {Kin C.} and Stephen Arnold",

note = "Funding Information: 7. ACKNOWLIDGEMENTS This research was sponsored by the U.S. Department of Energy, Office of Basic Energy Sciences, under contract DE-.ACO5-840R21400 with Martin Marietta Energy Systems. Publisher Copyright: {\textcopyright} 1993 Proceedings of SPIE - The International Society for Optical Engineering. All rights reserved.; Advances in Fluorescence Sensing Technology 1993 ; Conference date: 17-01-1993 Through 22-01-1993",

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doi = "10.1117/12.144732",

language = "English (US)",

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TY - JOUR

T1 - Detection of single molecules in microspheres

AU - Barnes, Michael D.

AU - Whiuen, William B.

AU - Ramsey, J. Michael

AU - Ng, Kin C.

AU - Arnold, Stephen

N1 - Funding Information: 7. ACKNOWLIDGEMENTS This research was sponsored by the U.S. Department of Energy, Office of Basic Energy Sciences, under contract DE-.ACO5-840R21400 with Martin Marietta Energy Systems. Publisher Copyright: © 1993 Proceedings of SPIE - The International Society for Optical Engineering. All rights reserved.

PY - 1993/5/18

Y1 - 1993/5/18

N2 - We have investigated the use of micron-sized liquid droplets as sample medium to detect single fluorescent molecules in solution. The use of microdroplets (5-15 jim diameter) offers several powerful advantages over single-molecule detection schemes involving measurements on bulk liquids where the probe volume is defined by the laser beam. In addition, cavity-quantum electrodynamical (QED) effects have been observed which influence both spontaneous emission rates and fluorescence yields of dye molecules in these microspheres.

AB - We have investigated the use of micron-sized liquid droplets as sample medium to detect single fluorescent molecules in solution. The use of microdroplets (5-15 jim diameter) offers several powerful advantages over single-molecule detection schemes involving measurements on bulk liquids where the probe volume is defined by the laser beam. In addition, cavity-quantum electrodynamical (QED) effects have been observed which influence both spontaneous emission rates and fluorescence yields of dye molecules in these microspheres.

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DO - 10.1117/12.144732

M3 - Conference article

AN - SCOPUS:85012467612

SN - 0277-786X

VL - 1885

SP - 458

EP - 465

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Advances in Fluorescence Sensing Technology 1993

Y2 - 17 January 1993 through 22 January 1993

ER -

Detection of single molecules in microspheres

Abstract

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