TY - JOUR
T1 - Determination of dna cleavage specificity by esperamicins
AU - Qiu Guo, Min Lu1
AU - Krishnan, Bala
AU - Golik, Jerzy
AU - Rosenberg, Ira E.
AU - Doyle, Terrence W.
AU - Kallenbach, Neville R.
N1 - Funding Information:
This research was supported by Grant CA-24101 from the National Institutes of Health.
Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 1991/10
Y1 - 1991/10
N2 - The esperamicins are members of a class of potent antitumor antibiotics that contain strained diacetylenic ring sytems capable of forming DNA -cleaving diradicals upon reaction with thiols. Here we show that the diacetylenic ring core itself determines the sequence specificity for scission of duplex DNA): esperamicin A1, and three products of hydrolysis of the glycon, esperamicins C, D, and E, are found to retain a common sequence preference. The sugar residues exert a strong influence on the cleavage efficiency, presumably by interacting nonspecifically with DNA The presence of a branch in the DNA is found locally to inhibit scission by esperamicins, and this effect is shown to be due to the core also.
AB - The esperamicins are members of a class of potent antitumor antibiotics that contain strained diacetylenic ring sytems capable of forming DNA -cleaving diradicals upon reaction with thiols. Here we show that the diacetylenic ring core itself determines the sequence specificity for scission of duplex DNA): esperamicin A1, and three products of hydrolysis of the glycon, esperamicins C, D, and E, are found to retain a common sequence preference. The sugar residues exert a strong influence on the cleavage efficiency, presumably by interacting nonspecifically with DNA The presence of a branch in the DNA is found locally to inhibit scission by esperamicins, and this effect is shown to be due to the core also.
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U2 - 10.1080/07391102.1991.10507913
DO - 10.1080/07391102.1991.10507913
M3 - Article
C2 - 1741964
AN - SCOPUS:0025989234
SN - 0739-1102
VL - 9
SP - 285
EP - 298
JO - Journal of Biomolecular Structure and Dynamics
JF - Journal of Biomolecular Structure and Dynamics
IS - 2
ER -