TY - JOUR
T1 - Determination of protein phosphorylation sites by mass spectrometry
T2 - A novel electrospray-based method
AU - Alverdi, Vera
AU - Di Pancrazio, Francesca
AU - Lippe, Giovanna
AU - Pucillo, Carlo
AU - Casetta, Bruno
AU - Esposito, Gennaro
PY - 2005
Y1 - 2005
N2 - Several methods are used to identify protein phosphorylation sites. We report a novel electrospray-based method for the determination of phosphorylation sites by mass spectrometry, using two different declustering potential values. This method allows one to obtain, with a single liquid chromatography/mass spectrometry (LC/MS) run, the pattern with either the phosphorylated or the unphosphorylated species of a protein tryptic digest, that can be further analyzed by tracing back the origin of each HPO 3-deprived form using the capabilities of tandem mass spectrometers.
AB - Several methods are used to identify protein phosphorylation sites. We report a novel electrospray-based method for the determination of phosphorylation sites by mass spectrometry, using two different declustering potential values. This method allows one to obtain, with a single liquid chromatography/mass spectrometry (LC/MS) run, the pattern with either the phosphorylated or the unphosphorylated species of a protein tryptic digest, that can be further analyzed by tracing back the origin of each HPO 3-deprived form using the capabilities of tandem mass spectrometers.
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U2 - 10.1002/rcm.2198
DO - 10.1002/rcm.2198
M3 - Article
C2 - 16235236
AN - SCOPUS:27944451013
SN - 0951-4198
VL - 19
SP - 3343
EP - 3348
JO - Rapid Communications in Mass Spectrometry
JF - Rapid Communications in Mass Spectrometry
IS - 22
ER -