Determining the genetic diversity of lactobacilli from the oral cavity

R. Yang, S. Argimon, Y. Li, X. Zhou, P. W. Caufield

Research output: Contribution to journalArticle

Abstract

Several methods for determining the diversity of Lactobacillus spp were evaluated with the purpose of developing a realistic approach for further studies. The patient population was comprised of young children with an oral disease called severe early childhood caries. The ultimate goal of these studies was to ascertain the role of lactobacilli in the caries process. To accomplish that goal, we evaluated several methods and approaches for determining diversity including AP-PCR, chromosomal DNA fingerprinting, denaturing gradient gel electrophoresis, and 16S rRNA gene sequencing. Central to these methods was the gathering and screening of isolates from cultivation medium. Using various estimates of diversity, we addressed the question as to how many isolates represent the overall diversity and how cultivation compares to non-cultivation techniques. Finally, we proposed a working approach for achieving the goals outlined framed by both practical constraints in terms of time, effort and efficacy while yielding a reliable outcome.

Original languageEnglish (US)
Pages (from-to)163-169
Number of pages7
JournalJournal of Microbiological Methods
Volume82
Issue number2
DOIs
StatePublished - Aug 2010

Keywords

  • 16S rRNA gene
  • AP-PCR
  • CDF
  • DGGE
  • Dental caries
  • Genotyping
  • Oral lactobacilli

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

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