Development and characterization of allospecific long-term human cytolytic T-cell lines

C. S. Reiss, M. E. Hemler, V. H. Englehard, J. W. Mier, J. L. Strominger, S. J. Burakoff

Research output: Contribution to journalArticle

Abstract

Two long-term human cytolytic T lymphocyte (CTL) lines (VE and JR), whose cytolytic activity was dependent upon both irradiated JY cells (the stimulating alloantigen) and T-cell growth factor, were established. These lines were monitored in culture for 6-8 months. Both lines were specific for HLA-A or B antigens or both and the JR line was allospecific for HLA-B7. These CTL lines killed specific target cells at an effector-to-target ratio of 0.4 (VE) or 0.08 (JR). All of the cells, which grow in suspension, rosetted with sheep erythrocytes and reacted with an antiserum specific for human T cells. The CTL line VE was used to raise rabbit antisera that immunoprecipitated two specific polypeptides (78,000 and 33,000 daltons) from labeled membranes of these CTL lines.

Original languageEnglish (US)
Pages (from-to)5432-5436
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume77
Issue number9 II
DOIs
StatePublished - 1980

ASJC Scopus subject areas

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