Development of a Body-on-a-Chip Using 3-D Microstructuring Technique

Yoshiki Kato, Yoshikazu Hirai, Ken Ichiro Kamei, Toshiyuki Tsuchiya, Osamu Tabata

Research output: Contribution to journalArticlepeer-review


IN vitro cell-based assay with human cells is getting attention since the accuracy of preclinical predictions of drug responses should be improved to reducing costly failures in clinical trials. In order to generate reliable predictions, we present a micro-engineered biomimetic systems "Body-on-a-Chip", to investigate the effects of drugs/metabolites on various organs by assembling a closed-loop medium circulation system on one microfluidic device. For three-dimensional (3-D) polymeric device fabrication, an advanced 3-D lithography with the process optimization method is applied to improve the current soft-lithography technique. Our Body-on-a-Chip is successfully applied to evaluate the effect of an anti-cancer drug (doxorubicin) on cell survival of human heart and liver cells.

Original languageEnglish (US)
Pages (from-to)229-236
Number of pages8
JournalIEEJ Transactions on Sensors and Micromachines
Issue number6
StatePublished - 2016


  • 3-dimensional microstructuring
  • Cell-based assay
  • Microfluidic device
  • PDMS
  • Pneumatic pump
  • Process optimization

ASJC Scopus subject areas

  • Mechanical Engineering
  • Electrical and Electronic Engineering


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