Development of immune cellular biosensing system for assessing chemicals on inducible nitric oxide synthase signaling activator

Ken Ichiro Kamei, Tetsuya Haruyama, Masayasu Mie, Yasuko Yanagida, Masuo Aizawa, Eiry Kobatake

Research output: Contribution to journalArticlepeer-review


An immune cellular biosensing system has been constructed to assess immunomodulating effects of chemicals. Production of nitric oxide in the immune cellular biosensing system was used as readout of an immune cellular response for assessing the immunomodulating effects of chemicals. The macrophage-like cell line RAW264.7, which has signaling pathways of inducible nitric oxide synthase, was employed in the cellular biosensing system. The immune cellular biosensing system consisted of a Pt counter electrode, an Ag/AgCl reference electrode, and a gold electrode onto which a polyion complex layer was coated to allow adherence of the RAW264.7 cells. As the results of evaluating effects of a polyion complex layer on cell viabilities by using WST-8 assay, the polyion complex layer did not affect RAW264.7 cells. The polyion-coated gold electrode could measure NO without the drawback of electrochemical interference that occurs with differential pulse voltammetry. The detection limit of the immune cellular biosensing system was 4.2nM released NO as measured by double potential step chronoamperometry. The potent immune activating abilities of lipopolysaccharide and interferon-γ could be assessed by the cellular biosensing system; NO release from cells was detected within 600ms.

Original languageEnglish (US)
Pages (from-to)75-81
Number of pages7
JournalAnalytical Biochemistry
Issue number1
StatePublished - Sep 1 2003


  • Cellular biosensing system
  • Inducible nitric oxide synthase
  • Interferon-γ
  • Lipopolysaccharide
  • Nitric oxide
  • Polyion complex

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Development of immune cellular biosensing system for assessing chemicals on inducible nitric oxide synthase signaling activator'. Together they form a unique fingerprint.

Cite this