TY - JOUR
T1 - Development of species-specific primers for detection of Streptococcus mutans in mixed bacterial samples
AU - Chen, Zhou
AU - Saxena, Deepak
AU - Caufield, Page W.
AU - Ge, Yao
AU - Wang, Minqi
AU - Li, Yihong
PY - 2007/7
Y1 - 2007/7
N2 - Streptococcus mutans is the major microbial pathogen associated with dental caries in children. The objectives of this study were to design and evaluate species-specific primers for the identification of S. mutans. Validation of the best primer set, Sm479F/R, was performed using seven S. mutans reference strains, 48 ATCC non-S. mutans strains, 92 S. mutans clinical isolates, DNA samples of S. mutans-Streptococcus sobrinus or S. mutans-Streptococcus sanguinis, and mixed bacterial DNA of saliva samples from 33 18-month-old children. All of the S. mutans samples tested positive, and no PCR products were amplified from members of the other streptococci or nonstreptococci strains examined. The lowest detection level for PCR was 10-2 ng of S. mutans DNA (c. 4.6 × 103 copies) in the test samples. The results of this study suggest that the Sm479F/R primer pair is highly specific and sensitive for identification of S. mutans in either purified or mixed DNA samples.
AB - Streptococcus mutans is the major microbial pathogen associated with dental caries in children. The objectives of this study were to design and evaluate species-specific primers for the identification of S. mutans. Validation of the best primer set, Sm479F/R, was performed using seven S. mutans reference strains, 48 ATCC non-S. mutans strains, 92 S. mutans clinical isolates, DNA samples of S. mutans-Streptococcus sobrinus or S. mutans-Streptococcus sanguinis, and mixed bacterial DNA of saliva samples from 33 18-month-old children. All of the S. mutans samples tested positive, and no PCR products were amplified from members of the other streptococci or nonstreptococci strains examined. The lowest detection level for PCR was 10-2 ng of S. mutans DNA (c. 4.6 × 103 copies) in the test samples. The results of this study suggest that the Sm479F/R primer pair is highly specific and sensitive for identification of S. mutans in either purified or mixed DNA samples.
KW - Dental caries
KW - PCR
KW - Species-specific primer
KW - Streptococcus mutans
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U2 - 10.1111/j.1574-6968.2007.00756.x
DO - 10.1111/j.1574-6968.2007.00756.x
M3 - Article
C2 - 17521362
AN - SCOPUS:34447502345
SN - 0378-1097
VL - 272
SP - 154
EP - 162
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 2
ER -