@article{c55656e279ba471eaafc25e3c616cd72,
title = "Developmental, cellular, and behavioral phenotypes in a mouse model of congenital hypoplasia of the dentate gyrus",
abstract = "In the hippocampus, a widely accepted model posits that the dentate gyrus improves learning and memory by enhancing discrimination between inputs. To test this model, we studied conditional knockout mice in which the vast majority of dentate granule cells (DGCs) fail to develop – including nearly all DGCs in the dorsal hippocampus – secondary to eliminating Wntless (Wls) in a subset of cortical progenitors with Gfap-Cre. Other cells in the Wlsfl/-;Gfap-Cre hippocampus were minimally affected, as determined by single nucleus RNA sequencing. CA3 pyramidal cells, the targets of DGC-derived mossy fibers, exhibited normal morphologies with a small reduction in the numbers of synaptic spines. Wlsfl/-;Gfap-Cre mice have a modest performance decrement in several complex spatial tasks, including active place avoidance. They were also modestly impaired in one simpler spatial task, finding a visible platform in the Morris water maze. These experiments support a role for DGCs in enhancing spatial learning and memory.",
author = "Amir Rattner and Terrillion, {Chantelle E.} and Claudia Jou and Tina Kleven and Hu, {Shun Felix} and John Williams and Zhipeng Hou and Manisha Aggarwal and Susumu Mori and Gloria Shin and Goff, {Loyal A.} and Witter, {Menno P.} and Mikhail Pletnikov and Fenton, {Andr{\'e} A.} and Jeremy Nathans",
note = "Funding Information: The authors thank Ruth Marx for assistance with hippocampal dissections; Linda Orzolek, Haiping Hao, and David Mohr for assistance with RNA sequencing; Grethe Olsen for assistance with Golgi stains; Yanshu Wang for advice; and James Knierim for advice and helpful comments on the manuscript. Supported by the Howard Hughes Medical Institute (AR, JW, JN), NIH R01NS105472 (CJ, SFH, AF), and a shared instrumentation grant S10 OD023472 (ZH, MA, SM), The Kavli Foundation Centre of Excellence – Centre for Neural Computation, Grant # 227769 of the Research Council of Norway, and the National Infrastructure scheme of the Research Council of Norway – NORBRAIN #197467 (MPW, TK), and NIH grants P50MH094268 and R01MH083728 (CET and MP). Funding Information: The authors thank Ruth Marx for assistance with hippocampal dissections; Linda Orzolek, Haiping Hao, and David Mohr for assistance with RNA sequencing; Grethe Olsen for assistance with Golgi stains; Yanshu Wang for advice; and James Knierim for advice and helpful comments on the manuscript. Supported by the Howard Hughes Medical Institute (AR, JW, JN), NIH R01NS105472 (CJ, SFH, AF), and a shared instrumentation grant S10 OD023472 (ZH, MA, SM), The Kavli Foundation Centre of Excellence ? Centre for Neural Computation, Grant # 227769 of the Research Council of Norway, and the National Infrastructure scheme of the Research Council of Norway ? NORBRAIN #197467 (MPW, TK), and NIH grants P50MH094268 and R01MH083728 (CET and MP). Publisher Copyright: {\textcopyright} Rattner et al.",
year = "2020",
month = oct,
doi = "10.7554/eLife.62766",
language = "English (US)",
volume = "9",
pages = "1--30",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
}