TY - JOUR
T1 - Developmental regulation and tissue-specific localization of calmodulin mRNA in the protochordate Cione intestinalis
AU - Gregorio, Anna Di
AU - Villani, Maria Grazia
AU - Locascio, Annamaria
AU - Ristoratore, Filomena
AU - Aniello, Francesco
AU - Branno, Margherita
PY - 1998
Y1 - 1998
N2 - A full-length cDNA encoding a highly conserved calmodulin was isolated from a cDNA library prepared from hatched larvae of the ascidian Ciona intestinalis. Sequence analysis has identified a 447 b.p. open reading frame, encoding a putative protein of 149 amino acid residues, with a predicted molecular weight of 16.8 kDa, showing 85-98% identity to known calmodulins. Northern blot analysis revealed a single transcript of about 0.8 kb in length, which was maternally expressed and progressively increased during development, until late tail-bud stage. Whole-mount in situ hybridizations, carried out on embryos at different stages of development, showed that starting from the neurula stage, the C. intestinalis calmodulin (Ci-CaM) expression became restricted to the neuroectoderm and that in larvae it was specifically detected in the nervous system.
AB - A full-length cDNA encoding a highly conserved calmodulin was isolated from a cDNA library prepared from hatched larvae of the ascidian Ciona intestinalis. Sequence analysis has identified a 447 b.p. open reading frame, encoding a putative protein of 149 amino acid residues, with a predicted molecular weight of 16.8 kDa, showing 85-98% identity to known calmodulins. Northern blot analysis revealed a single transcript of about 0.8 kb in length, which was maternally expressed and progressively increased during development, until late tail-bud stage. Whole-mount in situ hybridizations, carried out on embryos at different stages of development, showed that starting from the neurula stage, the C. intestinalis calmodulin (Ci-CaM) expression became restricted to the neuroectoderm and that in larvae it was specifically detected in the nervous system.
KW - Ascidian
KW - Calmodulin
KW - Ciona intestinalis
UR - http://www.scopus.com/inward/record.url?scp=0031846636&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031846636&partnerID=8YFLogxK
U2 - 10.1046/j.1440-169X.1998.t01-2-00003.x
DO - 10.1046/j.1440-169X.1998.t01-2-00003.x
M3 - Article
C2 - 9727352
AN - SCOPUS:0031846636
SN - 0012-1592
VL - 40
SP - 387
EP - 394
JO - Development Growth and Differentiation
JF - Development Growth and Differentiation
IS - 4
ER -