Developmental regulation and tissue-specific localization of calmodulin mRNA in the protochordate Cione intestinalis

Anna Di Gregorio, Maria Grazia Villani, Annamaria Locascio, Filomena Ristoratore, Francesco Aniello, Margherita Branno

Research output: Contribution to journalArticle

Abstract

A full-length cDNA encoding a highly conserved calmodulin was isolated from a cDNA library prepared from hatched larvae of the ascidian Ciona intestinalis. Sequence analysis has identified a 447 b.p. open reading frame, encoding a putative protein of 149 amino acid residues, with a predicted molecular weight of 16.8 kDa, showing 85-98% identity to known calmodulins. Northern blot analysis revealed a single transcript of about 0.8 kb in length, which was maternally expressed and progressively increased during development, until late tail-bud stage. Whole-mount in situ hybridizations, carried out on embryos at different stages of development, showed that starting from the neurula stage, the C. intestinalis calmodulin (Ci-CaM) expression became restricted to the neuroectoderm and that in larvae it was specifically detected in the nervous system.

Original languageEnglish (US)
Pages (from-to)387-394
Number of pages8
JournalDevelopment Growth and Differentiation
Volume40
Issue number4
DOIs
StatePublished - 1998

Keywords

  • Ascidian
  • Calmodulin
  • Ciona intestinalis

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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