Differential expression of IGF-I and insulin receptor isoforms in HPV positive and negative human cervical cancer cell lines

M. L. Serrano, M. Sánchez-Gómez, M. M. Bravo, S. Yakar, D. Leroith

Research output: Contribution to journalArticle

Abstract

Human papillomavirus (HPV) is the main risk factor for cervical cancer; however, some carcinomas occur in the absence of the virus. IGF-IR and an isoform of the insulin receptor, IR-A, play important roles in cancer. In this study we assessed the role of the IGF/insulin receptors in cervical cancer cell lines with different HPV status, SiHa (HPV positive), and C33a (HPV negative). Different patterns of receptor expression were found; while SiHa expressed IGF-IR, IR-A and IR-B, and IR/IGF-IR hybrid receptors, C33a cells expressed the IR-A only. Tyrosine phosphorylation of these receptors in response to their corresponding ligands correlated with the expression level of these receptors in the cell lines. Activation of PI3-K and MAPK pathways was revealed in both cell lines, however, no effects on proliferation, migration, or invasion were observed. Here we show that cervical cancer cell lines positive and negative for HPV differ in the type of insulin and IGF-1 receptors expressed. Additional studies are needed for characterization of the role of IR-A in cervical carcinogenesis.

Original languageEnglish (US)
Pages (from-to)25-31
Number of pages7
JournalTumor Diagnostik und Therapie
Volume30
Issue number1
DOIs
StatePublished - 2009

Keywords

  • C33a
  • Human papillomavirus (HPV)
  • IR-A
  • Insulin receptor (IR)
  • Insulin-like growth factor-I receptor (IGF-IR)
  • SiHa

ASJC Scopus subject areas

  • Oncology

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