Differential labeling with orotic acid and uridine in compensatory renal hypertrophy

J. S. Ross, D. Malamud, J. B. Caulfield, R. A. Malt

Research output: Contribution to journalArticlepeer-review


Using [5-3H]orotic acid and [5-3H]uridine as precursors, we compared the efficiency of labeling and the localization of labeled RNA during compensatory hypertrophy of the mouse kidney. [5-3H]orotic acid in tubules labeled RNA 15 times more intensely than [5-3H]uridine, presumably because of greater incorporation of orotic acid into tubular cells. Of the orotic acid label, 97% was in tubular cells, mostly in the proximal tubules. Only about 80% of the uridine label was in the tubules; the ratio in proximal tubules compared with that in distal tubules was 2:1. No changes in distribution within the nephron were produced during compensatory hypertrophy. [5-3H]uridine should be used as the precursor if generalized labeling is desired, but [5-3H]orotic acid is the better precursor of RNA for many studies of compensatory hypertrophy since it is more efficient and concentrates in the segments of greatest biologic activity.

Original languageEnglish (US)
Pages (from-to)952-954
Number of pages3
JournalAmerican Journal of Physiology
Issue number4
StatePublished - 1975

ASJC Scopus subject areas

  • Physiology (medical)

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