Diffusion-tensor imaging of human articular cartilage specimens with early signs of cartilage damage

José G. Raya, Gerd Melkus, Silvia Adam-Neumair, Olaf Dietrich, Elisabeth Mützel, Maximilian F. Reiser, Reinhard Putz, Thorsten Kirsch, Peter M. Jakob, Christian Glaser

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    Abstract

    Purpose: To assess the use of diffusion-tensor (DT) imaging of articular cartilage to detect and grade early cartilage damage in human specimens with early signs of cartilage damage. Materials and Methods: This study was approved by the institutional review board. Forty-three cartilage-on-bone samples drilled from 21 human patellae were examined with 17.6-T magnetic resonance (MR) imaging and a diffusion-weighted spinecho sequence (spatial resolution, 50 × 100 × 800 mm). Subsequently, samples underwent histologic analysis with safranin O staining. Cartilage damage on safranin O histologic slides was quantified with Osteoarthritis Research Society International (OARSI) grades; grades ranged from 0 (healthy) to 6 (bone remodeling). Maps of longitudinal diffusivity (λ1), transverse diffusivity (λ t), mean diffusivity (MD), and fractional anisotropy (FA) were calculated. Cartilage was segmented, and region of interest (ROI) analysis was performed and compared with histologic findings. Significant differences in MR parameters between the OARSI groups were assessed with the Tukey test. The value of DT imaging in the diagnosis and grading of cartilage damage was assessed with logistic regression analysis. Results: Samples had OARSI grades of 0 (n = 14), 1 (n = 11), 2 (n = 12), 3 (n = 4), and 4 (n = 2). Samples with an OARSI grade greater than 0 had significantly increased ll, lt, and MD (7%-25% increase) in the superficial cartilage growing deeper into cartilage with increasing OARSI grade. Samples with an OARSI grade greater than 0 showed significantly decreased FA in the deep cartilage (225% to 235% decrease), suggesting that changes in the collagen architecture may occur early in cartilage degradation. DTI showed excellent performance in the detection of cartilage damage (accuracy, 0.95; 41 of 43 samples) and good performance in the grading of cartilage damage (accuracy, 0.74; 32 of 43 samples). Conclusion: DT imaging of articular cartilage can enable physicians to detect and grade early cartilage damage.

    Original languageEnglish (US)
    Pages (from-to)831-841
    Number of pages11
    JournalRadiology
    Volume266
    Issue number3
    DOIs
    StatePublished - Mar 2013

    ASJC Scopus subject areas

    • Radiology Nuclear Medicine and imaging

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    Raya, J. G., Melkus, G., Adam-Neumair, S., Dietrich, O., Mützel, E., Reiser, M. F., Putz, R., Kirsch, T., Jakob, P. M., & Glaser, C. (2013). Diffusion-tensor imaging of human articular cartilage specimens with early signs of cartilage damage. Radiology, 266(3), 831-841. https://doi.org/10.1148/radiol.12120954