288Digital microfluidics refers to the manipulation of small, discrete quantities of fluids [1,2]. This paradigm runs counter to older, more established microfluidic design methods, which rely on continuous flow channels driven by micropumps and controlled by microvalves [3,4]. The discrete nature of digital microfluidics lends itself nicely to design automation techniques; many methods for high-level synthesis , design-for-test , and error recovery [7,8] have been demonstrated, leading to increased ease-of-use, throughput and reliability. Digital microfluidic biochips (DMFBs) have thus emerged as one of the most promising techniques for realizing the lab-on-a-chip.
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