@article{6ae19c13dc7c451186270b07458ae079,
title = "Dipyridamole-loaded 3D-printed bioceramic scaffolds stimulate pediatric bone regeneration in vivo without disruption of craniofacial growth through facial maturity",
abstract = "This study investigates a comprehensive model of bone regeneration capacity of dypiridamole-loaded 3D-printed bioceramic (DIPY-3DPBC) scaffolds composed of 100% beta-tricalcium phosphate (β –TCP) in an immature rabbit model through the time of facial maturity. The efficacy of this construct was compared to autologous bone graft, the clinical standard of care in pediatric craniofacial reconstruction, with attention paid to volume of regenerated bone by 3D reconstruction, histologic and mechanical properties of regenerated bone, and long-term safety regarding potential craniofacial growth restriction. Additionally, long-term degradation of scaffold constructs was evaluated. At 24 weeks in vivo, DIPY-3DPBC scaffolds demonstrated volumetrically significant osteogenic regeneration of calvarial and alveolar defects comparable to autogenous bone graft with favorable biodegradation of the bioactive ceramic component in vivo. Characterization of regenerated bone reveals osteogenesis of organized, vascularized bone with histologic and mechanical characteristics comparable to native bone. Radiographic and histologic analyses were consistent with patent craniofacial sutures. Lastly, through application of 3D morphometric facial surface analysis, our results support that DIPY-3DPBC scaffolds do not cause premature closure of sutures and preserve normal craniofacial growth. Based on this novel evaluation model, this DIPY-3DPBC scaffold strategy is a promising candidate as a safe, efficacious pediatric bone tissue engineering strategy.",
author = "Wang, {Maxime M.} and Flores, {Roberto L.} and Lukasz Witek and Andrea Torroni and Amel Ibrahim and Zhong Wang and Liss, {Hannah A.} and Cronstein, {Bruce N.} and Lopez, {Christopher D.} and Maliha, {Samantha G.} and Coelho, {Paulo G.}",
note = "Funding Information: This work was supported by the National Institutes of Health [R21/R33 HD090664-01 “Use of 3D Printing for Creation of Implantable Pediatric Devices”], and B.C. and P.C. were supported by grants NIH [R01 AR068593] and NYU-H+H Clinical and Translational Science Institute Grant (1UL1TR001445). Dr. Ibrahim was supported by a Royal College of Surgeons Fulbright Scholarship. We would also like to thank Prof. Hammond and Dr. Suttie at University of Oxford for their development of the software used in morphometric analysis. Thank you also to Gregory D. Kurgansky, Ricardo Rodriguez Colon, and Cristobal Rivera for their contributions and support. Funding Information: B.C. has patented the use of dipyridamole-loaded 3D printed biodegradable scaffolds for the treatment of critical bone defects. P.C. has patented the 3D printing device configuration for bone regeneration. B.C. and P.C. were funded by the National Institutes of Health [Grand #: R21/R33 HD090664-01 “Use of 3D Printing for Creation of Implantable Pediatric Devices”] B.C. and P.C. were further supported by grants NIH [R01 AR068593] and NYU-H+H Clinical and Translational Science Institute Grant (1UL1TR001445). M.W., R.F., L.W., A.T., A.I., Z.W., H.L., C.L. and S.M. declare no potential conflict of interest.",
year = "2019",
month = dec,
day = "1",
doi = "10.1038/s41598-019-54726-6",
language = "English (US)",
volume = "9",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",
}