Direct measurement of interactions between adsorbed protein layers using an atomic force microscope

W. Richard Bowen, Nidal Hilal, Robert W. Lovitt, Chris J. Wright

Research output: Contribution to journalArticlepeer-review

Abstract

An atomic force microscope (AFM) in conjunction with the colloid probe technique has been used to measure directly the interaction of adsorbed layers of the protein bovine serum albumin (BSA). The BSA was adsorbed on both a silica colloid probe and a silica surface. Measurements of force- distance curves were made at various salt concentrations and pHs. The measured force-distance curves were in good quantitative agreement with predictions based on the DLVO theory using zeta potentials (outer Helmholtz plane potentials) calculated for BSA from an independently validated site- binding-site-dissociation surface model. The paper hence provides a direct confirmation that the AFM colloid probe technique can provide a useful means of directly quantifying the interaction of biological macromolecules.

Original languageEnglish (US)
Pages (from-to)348-352
Number of pages5
JournalJournal of Colloid And Interface Science
Volume197
Issue number2
DOIs
StatePublished - Jan 15 1998

Keywords

  • Atomic force microscope
  • BSA
  • Colloid probe
  • DLVO

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Surfaces, Coatings and Films
  • Colloid and Surface Chemistry

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