Direct measurement of the force of adhesion of a single biological cell using an atomic force microscope

W. Richard Bowen; Nidal Hilal; Robert W. Lovitt; Chris J. Wright

doi:10.1016/S0927-7757(97)00243-4

Direct measurement of the force of adhesion of a single biological cell using an atomic force microscope

W. Richard Bowen, Nidal Hilal, Robert W. Lovitt, Chris J. Wright

Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

A fundamental understanding of factors controlling adhesion and the subsequent development of adhesion-free surfaces would benefit greatly from direct measurements of the strength of the adhesive interactions. The key to the technique is the immobilization of a single particle at the end of a cantilever allowing measurement of the interactions between that particle and any surface of interest. Such colloid probes with particles in the size range of 1-5 μm were used to study long-range interactions in solution and also in a few cases the adhesion of inorganic particles. The first use of a single, living, immobilized cell as a cell probe for the study of cell-surface adhesion is reported.

Original language	English (US)
Pages (from-to)	231-234
Number of pages	4
Journal	Colloids and Surfaces A: Physicochemical and Engineering Aspects
Volume	136
Issue number	1-2
DOIs	https://doi.org/10.1016/S0927-7757(97)00243-4
State	Published - Apr 30 1998

Keywords

AFM
Adhesion
Cell probe

ASJC Scopus subject areas

Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

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10.1016/S0927-7757(97)00243-4

Cite this

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title = "Direct measurement of the force of adhesion of a single biological cell using an atomic force microscope",

abstract = "A fundamental understanding of factors controlling adhesion and the subsequent development of adhesion-free surfaces would benefit greatly from direct measurements of the strength of the adhesive interactions. The key to the technique is the immobilization of a single particle at the end of a cantilever allowing measurement of the interactions between that particle and any surface of interest. Such colloid probes with particles in the size range of 1-5 μm were used to study long-range interactions in solution and also in a few cases the adhesion of inorganic particles. The first use of a single, living, immobilized cell as a cell probe for the study of cell-surface adhesion is reported.",

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author = "Bowen, {W. Richard} and Nidal Hilal and Lovitt, {Robert W.} and Wright, {Chris J.}",

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T1 - Direct measurement of the force of adhesion of a single biological cell using an atomic force microscope

AU - Bowen, W. Richard

AU - Hilal, Nidal

AU - Lovitt, Robert W.

AU - Wright, Chris J.

N1 - Funding Information: This work was funded by the UK Engineering and Physical Sciences Research Council.

PY - 1998/4/30

Y1 - 1998/4/30

N2 - A fundamental understanding of factors controlling adhesion and the subsequent development of adhesion-free surfaces would benefit greatly from direct measurements of the strength of the adhesive interactions. The key to the technique is the immobilization of a single particle at the end of a cantilever allowing measurement of the interactions between that particle and any surface of interest. Such colloid probes with particles in the size range of 1-5 μm were used to study long-range interactions in solution and also in a few cases the adhesion of inorganic particles. The first use of a single, living, immobilized cell as a cell probe for the study of cell-surface adhesion is reported.

AB - A fundamental understanding of factors controlling adhesion and the subsequent development of adhesion-free surfaces would benefit greatly from direct measurements of the strength of the adhesive interactions. The key to the technique is the immobilization of a single particle at the end of a cantilever allowing measurement of the interactions between that particle and any surface of interest. Such colloid probes with particles in the size range of 1-5 μm were used to study long-range interactions in solution and also in a few cases the adhesion of inorganic particles. The first use of a single, living, immobilized cell as a cell probe for the study of cell-surface adhesion is reported.

KW - AFM

KW - Adhesion

KW - Cell probe

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Direct measurement of the force of adhesion of a single biological cell using an atomic force microscope

Abstract

Keywords

ASJC Scopus subject areas

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