Discovery of Stable and Selective Antibody Mimetics from Combinatorial Libraries of Polyvalent, Loop-Functionalized Peptoid Nanosheets

Jae Hong Kim, Samuel C. Kim, Mark A. Kline, Elissa M. Grzincic, Blakely W. Tresca, Joshua Cardiel, Mohsen Karbaschi, Dilani C. Dehigaspitiya, Yulin Chen, Venkatareddy Udumula, Tengyue Jian, Daniel J. Murray, Lisa Yun, Michael D. Connolly, Jianfang Liu, Gang Ren, Chun Long Chen, Kent Kirshenbaum, Adam R. Abate, Ronald N. Zuckermann

Research output: Contribution to journalArticle

Abstract

The ability of antibodies to bind a wide variety of analytes with high specificity and high affinity makes them ideal candidates for therapeutic and diagnostic applications. However, the poor stability and high production cost of antibodies have prompted exploration of a variety of synthetic materials capable of specific molecular recognition. Unfortunately, it remains a fundamental challenge to create a chemically diverse population of protein-like, folded synthetic nanostructures with defined molecular conformations in water. Here we report the synthesis and screening of combinatorial libraries of sequence-defined peptoid polymers engineered to fold into ordered, supramolecular nanosheets displaying a high spatial density of diverse, conformationally constrained peptoid loops on their surface. These polyvalent, loop-functionalized nanosheets were screened using a homogeneous Förster resonance energy transfer (FRET) assay for binding to a variety of protein targets. Peptoid sequences were identified that bound to the heptameric protein, anthrax protective antigen, with high avidity and selectivity. These nanosheets were shown to be resistant to proteolytic degradation, and the binding was shown to be dependent on the loop display density. This work demonstrates that key aspects of antibody structure and function-the creation of multivalent, combinatorial chemical diversity within a well-defined folded structure-can be realized with completely synthetic materials. This approach enables the rapid discovery of biomimetic affinity reagents that combine the durability of synthetic materials with the specificity of biomolecular materials.

Original languageEnglish (US)
Pages (from-to)185-195
Number of pages11
JournalACS nano
Volume14
Issue number1
DOIs
StatePublished - Jan 28 2020

Keywords

  • bioinspired polymers
  • combinatorial display
  • multivalent molecular recognition
  • protein-mimetic materials
  • two-dimensional nanomaterials

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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  • Cite this

    Kim, J. H., Kim, S. C., Kline, M. A., Grzincic, E. M., Tresca, B. W., Cardiel, J., Karbaschi, M., Dehigaspitiya, D. C., Chen, Y., Udumula, V., Jian, T., Murray, D. J., Yun, L., Connolly, M. D., Liu, J., Ren, G., Chen, C. L., Kirshenbaum, K., Abate, A. R., & Zuckermann, R. N. (2020). Discovery of Stable and Selective Antibody Mimetics from Combinatorial Libraries of Polyvalent, Loop-Functionalized Peptoid Nanosheets. ACS nano, 14(1), 185-195. https://doi.org/10.1021/acsnano.9b07498