TY - JOUR
T1 - Disposable polydimethylsiloxane/silicon hybrid chips for protein detection
AU - Li, Shifeng
AU - Fozdar, David
AU - Shao, Dongbing
AU - Chen, Shaochen
AU - Floriane, Pierre N.
AU - Christodoulides, Nicolaos
AU - Ali, Mehnaaz F.
AU - Dharshan, Priya
AU - McDevitt, John T.
AU - Neikirk, Dean
PY - 2004
Y1 - 2004
N2 - This paper presents disposable protein analysis chips with single and multiple chambers - constructed from poly (dimethylsiloxane) (PDMS) and silicon. The chips are composed of a multilayer stack of PDMS layers that sandwich a silicon microchip. This inner silicon chip features an etched array of microcavities hosting agarose beads. The sample is introduced into the fluid network in the top PDMS layer where it is directed to the bead chamber. After reaction of the analyte with the probe beads, signal generated on the beads is captured with a CCD camera, digitally processed, and analyzed. An established bead-based fluorescent assay for C-reactive protein (CRP) was used here to characterize these hybrid chips. The detection limit of the single chamber protein chip was found to be Ing/mL. Additionally, using the back pressure compensation method, the signals from each of the four-chamber chip were found to be within 10% of each other. Moreover, the fabrication of the multiple-chamber chip may increase throughput and multiplex assay capacity.
AB - This paper presents disposable protein analysis chips with single and multiple chambers - constructed from poly (dimethylsiloxane) (PDMS) and silicon. The chips are composed of a multilayer stack of PDMS layers that sandwich a silicon microchip. This inner silicon chip features an etched array of microcavities hosting agarose beads. The sample is introduced into the fluid network in the top PDMS layer where it is directed to the bead chamber. After reaction of the analyte with the probe beads, signal generated on the beads is captured with a CCD camera, digitally processed, and analyzed. An established bead-based fluorescent assay for C-reactive protein (CRP) was used here to characterize these hybrid chips. The detection limit of the single chamber protein chip was found to be Ing/mL. Additionally, using the back pressure compensation method, the signals from each of the four-chamber chip were found to be within 10% of each other. Moreover, the fabrication of the multiple-chamber chip may increase throughput and multiplex assay capacity.
KW - Assay
KW - C-reactive protein
KW - Disposable
KW - Poly (dimethylsiloxane)
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U2 - 10.1115/IMECE2004-62170
DO - 10.1115/IMECE2004-62170
M3 - Conference article
AN - SCOPUS:21644473757
SN - 1096-665X
SP - 331
EP - 337
JO - American Society of Mechanical Engineers, Micro-Electro Mechanical Systems Division, (Publications) MEMS
JF - American Society of Mechanical Engineers, Micro-Electro Mechanical Systems Division, (Publications) MEMS
M1 - IMECE2004-62170
T2 - 2004 ASME International Mechanical Engineering Congress and Exposition, IMECE
Y2 - 13 November 2004 through 19 November 2004
ER -