Disposable polydimethylsiloxane/silicon hybrid chips for protein detection

Shifeng Li, David Fozdar, Dongbing Shao, Shaochen Chen, Pierre N. Floriane, Nicolaos Christodoulides, Mehnaaz F. Ali, Priya Dharshan, John T. McDevitt, Dean Neikirk

Research output: Contribution to journalConference articlepeer-review


This paper presents disposable protein analysis chips with single and multiple chambers - constructed from poly (dimethylsiloxane) (PDMS) and silicon. The chips are composed of a multilayer stack of PDMS layers that sandwich a silicon microchip. This inner silicon chip features an etched array of microcavities hosting agarose beads. The sample is introduced into the fluid network in the top PDMS layer where it is directed to the bead chamber. After reaction of the analyte with the probe beads, signal generated on the beads is captured with a CCD camera, digitally processed, and analyzed. An established bead-based fluorescent assay for C-reactive protein (CRP) was used here to characterize these hybrid chips. The detection limit of the single chamber protein chip was found to be Ing/mL. Additionally, using the back pressure compensation method, the signals from each of the four-chamber chip were found to be within 10% of each other. Moreover, the fabrication of the multiple-chamber chip may increase throughput and multiplex assay capacity.

Original languageEnglish (US)
Article numberIMECE2004-62170
Pages (from-to)331-337
Number of pages7
JournalAmerican Society of Mechanical Engineers, Micro-Electro Mechanical Systems Division, (Publications) MEMS
StatePublished - 2004
Event2004 ASME International Mechanical Engineering Congress and Exposition, IMECE - Anaheim, CA, United States
Duration: Nov 13 2004Nov 19 2004


  • Assay
  • C-reactive protein
  • Disposable
  • Poly (dimethylsiloxane)

ASJC Scopus subject areas

  • General Engineering


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