TY - JOUR
T1 - Disruption of IFN-γ - Mediated antiviral activity in neurons
T2 - The role of cannabinoids
AU - Herrera, R. Antonio
AU - Oved, Joseph H.
AU - Reiss, Carol Shoshkes
PY - 2008/6/1
Y1 - 2008/6/1
N2 - Interferon-γ (IFN-γ) has potent antiviral activity in neurons which is affected by the production of nitric oxide (NO). This study examines the interactions between cannabinoid receptor-1 (CB1), IFNγ-induced pathways, and inhibition of vesicular stomatitis virus (VSV) replication in neuronal cells. CB1 is abundantly expressed in neurons of the CNS and the NB41A3 neuroblastoma cell line. CB1 activation of NB41A3 cells by the synthetic cannabinoid, WIN55,212-2, is associated with an inhibition of Ca2+ mobilization, leading to diminished nitric oxide synthase (NOS)-1 activity and the production of NO, in vitro. This ultimately results in antagonism of IFN-γ-mediated antiviral activity and enhanced viral replication. Therefore, activation of cells expressing CB1 by endogenous (or exogenous) ligands may contribute to decreased inflammation and to increased viral replication in neurons and disease in the CNS.
AB - Interferon-γ (IFN-γ) has potent antiviral activity in neurons which is affected by the production of nitric oxide (NO). This study examines the interactions between cannabinoid receptor-1 (CB1), IFNγ-induced pathways, and inhibition of vesicular stomatitis virus (VSV) replication in neuronal cells. CB1 is abundantly expressed in neurons of the CNS and the NB41A3 neuroblastoma cell line. CB1 activation of NB41A3 cells by the synthetic cannabinoid, WIN55,212-2, is associated with an inhibition of Ca2+ mobilization, leading to diminished nitric oxide synthase (NOS)-1 activity and the production of NO, in vitro. This ultimately results in antagonism of IFN-γ-mediated antiviral activity and enhanced viral replication. Therefore, activation of cells expressing CB1 by endogenous (or exogenous) ligands may contribute to decreased inflammation and to increased viral replication in neurons and disease in the CNS.
UR - http://www.scopus.com/inward/record.url?scp=45749113356&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=45749113356&partnerID=8YFLogxK
U2 - 10.1089/vim.2007.0109
DO - 10.1089/vim.2007.0109
M3 - Article
C2 - 18570588
AN - SCOPUS:45749113356
SN - 0882-8245
VL - 21
SP - 141
EP - 152
JO - Viral Immunology
JF - Viral Immunology
IS - 2
ER -