@article{2aeed47f340641199dd89b1cb03f4743,
title = "Dissection of affinity captured LINE-1 macromolecular complexes",
abstract = "Long Interspersed Nuclear Element-1 (LINE-1, L1) is a mobile genetic element active in human genomes. L1-encoded ORF1 and ORF2 proteins bind L1 RNAs, forming ribonucleoproteins (RNPs). These RNPs interact with diverse host proteins, some repressive and others required for the L1 lifecycle. Using differential affinity purifications, quantitative mass spectrometry, and next generation RNA sequencing, we have characterized the proteins and nucleic acids associated with distinctive, enzymatically active L1 macromolecular complexes. Among them, we describe a cytoplasmic intermediate that we hypothesize to be the canonical ORF1p/ORF2p/L1-RNAcontaining RNP, and we describe a nuclear population containing ORF2p, but lacking ORF1p, which likely contains host factors participating in target-primed reverse transcription.",
author = "Taylor, {Martin S.} and Ilya Altukhov and Molloy, {Kelly R.} and Paolo Mita and Hua Jiang and Adney, {Emily M.} and Aleksandra Wudzinska and Sana Badri and Dmitry Ischenko and George Eng and Burns, {Kathleen H.} and David Feny{\"o} and Chait, {Brian T.} and Dmitry Alexeev and Rout, {Michael P.} and Boeke, {Jef D.} and John LaCava",
note = "Funding Information: This work was supported in part by National Institutes of Health (NIH) grants P41GM109824 (to MPR), P41GM103314 (to BTC), P50GM107632 (to JDB), and R01GM126170 (to JL), and by the 5-100 Russian Academic Excellence Program. The mass spectrometric analysis of proteins co-captured with chromatin associated ORF2p (Supplementary file 3) was conducted within the NYU School of Medicine Proteomics Resource Lab, which is partially supported by the Laura and Isaac Perlmutter Cancer Center Support Grant, NIH P30CA16087, and NIH 1S10OD010582. RNA sequence library preparation and next-generation sequencing was carried out by The Rockefeller University Genomics Resource Center. Peptide synthesis was performed by Henry Zebroski at The Rockefeller University Proteomics Resource Center. We thank Carolyn Machamer for advice and resources supporting fluorescence microscopy, and Lixin Dai for assistance with LEAP assays. This paper is subject to the NIH Public Access Policy. The authors declare no conflicts of interest. Publisher Copyright: {\textcopyright} Taylor et al.",
year = "2018",
month = jan,
day = "8",
doi = "10.7554/eLife.30094",
language = "English (US)",
volume = "7",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
}