Dissection of affinity captured LINE-1 macromolecular complexes

Martin S. Taylor, Ilya Altukhov, Kelly R. Molloy, Paolo Mita, Hua Jiang, Emily M. Adney, Aleksandra Wudzinska, Sana Badri, Dmitry Ischenko, George Eng, Kathleen H. Burns, David Fenyö, Brian T. Chait, Dmitry Alexeev, Michael P. Rout, Jef D. Boeke, John LaCava

    Research output: Contribution to journalArticle

    Abstract

    Long Interspersed Nuclear Element-1 (LINE-1, L1) is a mobile genetic element active in human genomes. L1-encoded ORF1 and ORF2 proteins bind L1 RNAs, forming ribonucleoproteins (RNPs). These RNPs interact with diverse host proteins, some repressive and others required for the L1 lifecycle. Using differential affinity purifications, quantitative mass spectrometry, and next generation RNA sequencing, we have characterized the proteins and nucleic acids associated with distinctive, enzymatically active L1 macromolecular complexes. Among them, we describe a cytoplasmic intermediate that we hypothesize to be the canonical ORF1p/ORF2p/L1-RNAcontaining RNP, and we describe a nuclear population containing ORF2p, but lacking ORF1p, which likely contains host factors participating in target-primed reverse transcription.

    Original languageEnglish (US)
    Article numbere30094
    JournaleLife
    Volume7
    DOIs
    StatePublished - Jan 8 2018

    ASJC Scopus subject areas

    • Neuroscience(all)
    • Immunology and Microbiology(all)
    • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Taylor, M. S., Altukhov, I., Molloy, K. R., Mita, P., Jiang, H., Adney, E. M., Wudzinska, A., Badri, S., Ischenko, D., Eng, G., Burns, K. H., Fenyö, D., Chait, B. T., Alexeev, D., Rout, M. P., Boeke, J. D., & LaCava, J. (2018). Dissection of affinity captured LINE-1 macromolecular complexes. eLife, 7, [e30094]. https://doi.org/10.7554/eLife.30094