DNA binding by single HMG box model proteins

Hong Xin, Susann Taudte, Neville R. Kallenbach, M. Paullin Limbach, Richard S. Zitomer

Research output: Contribution to journalArticle

Abstract

The HMG1/2 family is a large group of proteins that share a conserved sequence of ~80 amino acids rich in basic, aromatic and proline side chains, referred to as an HMG box. Previous studies show that HMG boxes can bind to DNA in a structure-specific manner. To define the basis for DNA recognition by HMG boxes, we characterize the interaction of two model HMG boxes, one a structure-specific box, rHMGb from the rat HMG1 protein, the other a sequence-specific box, Rox1 from yeast, with oligodeoxynucleotide substrates. Both proteins interact with single-stranded oligonucleotides in this study to form 1:1 complexes. The stoichiometry of binding of rHMGb to duplex or branched DNAs differs: for a 16mer duplex we find a weak 2:1 complex, while a 4:1 protein:DNA complex is detected with a four-way DNA junction of 16mers in the presence of Mg2+. In the case of the sequence-specific Rox1 protein we find tight 1:1 and 2:1 complexes with its cognate duplex sequence and again a 4:1 complex with four-way branched DNA. If the DNA branching is reduced to three arms, both proteins form 3:1 complexes. We believe that these multimeric complexes are relevant for HMG1/2 proteins in vivo, since Mg2+ is present in the nucleus and these proteins are expressed at a very high level.

Original languageEnglish (US)
Pages (from-to)4044-4050
Number of pages7
JournalNucleic acids research
Volume28
Issue number20
DOIs
StatePublished - Oct 15 2000

ASJC Scopus subject areas

  • Genetics

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    Xin, H., Taudte, S., Kallenbach, N. R., Limbach, M. P., & Zitomer, R. S. (2000). DNA binding by single HMG box model proteins. Nucleic acids research, 28(20), 4044-4050. https://doi.org/10.1093/nar/28.20.4044