TY - JOUR
T1 - DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle
AU - Mikheikin, Andrey
AU - Olsen, Anita
AU - Leslie, Kevin
AU - Russell-Pavier, Freddie
AU - Yacoot, Andrew
AU - Picco, Loren
AU - Payton, Oliver
AU - Toor, Amir
AU - Chesney, Alden
AU - Gimzewski, James K.
AU - Mishra, Bud
AU - Reed, Jason
N1 - Funding Information:
Funding for this work was provided by National Institutes of Health grant R01GM094388 to J.R., B.M. and J.K.G. and grant R01CA185189 to J.R. Services in support of the research project was provided by the VCU Massey Cancer Center Tissue and Data Acquisition and Analysis Core, supported, in part, with funding from NIH-NCI Cancer Center Support Grant P30 CA016059.
Publisher Copyright:
© 2017 The Author(s).
PY - 2017/12/1
Y1 - 2017/12/1
N2 - Progress in whole-genome sequencing using short-read (e.g., <150 bp), next-generation sequencing technologies has reinvigorated interest in high-resolution physical mapping to fill technical gaps that are not well addressed by sequencing. Here, we report two technical advances in DNA nanotechnology and single-molecule genomics: (1) we describe a labeling technique (CRISPR-Cas9 nanoparticles) for high-speed AFM-based physical mapping of DNA and (2) the first successful demonstration of using DVD optics to image DNA molecules with high-speed AFM. As a proof of principle, we used this new "nanomapping" method to detect and map precisely BCL2-IGH translocations present in lymph node biopsies of follicular lymphoma patents. This HS-AFM "nanomapping" technique can be complementary to both sequencing and other physical mapping approaches.
AB - Progress in whole-genome sequencing using short-read (e.g., <150 bp), next-generation sequencing technologies has reinvigorated interest in high-resolution physical mapping to fill technical gaps that are not well addressed by sequencing. Here, we report two technical advances in DNA nanotechnology and single-molecule genomics: (1) we describe a labeling technique (CRISPR-Cas9 nanoparticles) for high-speed AFM-based physical mapping of DNA and (2) the first successful demonstration of using DVD optics to image DNA molecules with high-speed AFM. As a proof of principle, we used this new "nanomapping" method to detect and map precisely BCL2-IGH translocations present in lymph node biopsies of follicular lymphoma patents. This HS-AFM "nanomapping" technique can be complementary to both sequencing and other physical mapping approaches.
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U2 - 10.1038/s41467-017-01891-9
DO - 10.1038/s41467-017-01891-9
M3 - Article
C2 - 29162844
AN - SCOPUS:85034746578
VL - 8
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
M1 - 1665
ER -