DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle

Andrey Mikheikin; Anita Olsen; Kevin Leslie; Freddie Russell-Pavier; Andrew Yacoot; Loren Picco; Oliver Payton; Amir Toor; Alden Chesney; James K. Gimzewski; Bud Mishra; Jason Reed

doi:10.1038/s41467-017-01891-9

DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle

Andrey Mikheikin, Anita Olsen, Kevin Leslie, Freddie Russell-Pavier, Andrew Yacoot, Loren Picco, Oliver Payton, Amir Toor, Alden Chesney, James K. Gimzewski, Bud Mishra, Jason Reed

Computer Science

Research output: Contribution to journal › Article

Abstract

Progress in whole-genome sequencing using short-read (e.g., <150 bp), next-generation sequencing technologies has reinvigorated interest in high-resolution physical mapping to fill technical gaps that are not well addressed by sequencing. Here, we report two technical advances in DNA nanotechnology and single-molecule genomics: (1) we describe a labeling technique (CRISPR-Cas9 nanoparticles) for high-speed AFM-based physical mapping of DNA and (2) the first successful demonstration of using DVD optics to image DNA molecules with high-speed AFM. As a proof of principle, we used this new "nanomapping" method to detect and map precisely BCL2-IGH translocations present in lymph node biopsies of follicular lymphoma patents. This HS-AFM "nanomapping" technique can be complementary to both sequencing and other physical mapping approaches.

Original language	English (US)
Article number	1665
Journal	Nature communications
Volume	8
Issue number	1
DOIs	https://doi.org/10.1038/s41467-017-01891-9
State	Published - Dec 1 2017

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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Mikheikin, A., Olsen, A., Leslie, K., Russell-Pavier, F., Yacoot, A., Picco, L., Payton, O., Toor, A., Chesney, A., Gimzewski, J. K., Mishra, B., & Reed, J. (2017). DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle. Nature communications, 8(1), [1665]. https://doi.org/10.1038/s41467-017-01891-9

DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle. / Mikheikin, Andrey; Olsen, Anita; Leslie, Kevin; Russell-Pavier, Freddie; Yacoot, Andrew; Picco, Loren; Payton, Oliver; Toor, Amir; Chesney, Alden; Gimzewski, James K.; Mishra, Bud; Reed, Jason.

In: Nature communications, Vol. 8, No. 1, 1665, 01.12.2017.

Research output: Contribution to journal › Article

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