DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle

Andrey Mikheikin; Anita Olsen; Kevin Leslie; Freddie Russell-Pavier; Andrew Yacoot; Loren Picco; Oliver Payton; Amir Toor; Alden Chesney; James K. Gimzewski; Bud Mishra; Jason Reed

doi:10.1038/s41467-017-01891-9

DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle

Andrey Mikheikin, Anita Olsen, Kevin Leslie, Freddie Russell-Pavier, Andrew Yacoot, Loren Picco, Oliver Payton, Amir Toor, Alden Chesney, James K. Gimzewski, Bud Mishra, Jason Reed

Computer Science

Research output: Contribution to journal › Article › peer-review

Abstract

Progress in whole-genome sequencing using short-read (e.g., <150 bp), next-generation sequencing technologies has reinvigorated interest in high-resolution physical mapping to fill technical gaps that are not well addressed by sequencing. Here, we report two technical advances in DNA nanotechnology and single-molecule genomics: (1) we describe a labeling technique (CRISPR-Cas9 nanoparticles) for high-speed AFM-based physical mapping of DNA and (2) the first successful demonstration of using DVD optics to image DNA molecules with high-speed AFM. As a proof of principle, we used this new "nanomapping" method to detect and map precisely BCL2-IGH translocations present in lymph node biopsies of follicular lymphoma patents. This HS-AFM "nanomapping" technique can be complementary to both sequencing and other physical mapping approaches.

Original language	English (US)
Article number	1665
Journal	Nature communications
Volume	8
Issue number	1
DOIs	https://doi.org/10.1038/s41467-017-01891-9
State	Published - Dec 1 2017

ASJC Scopus subject areas

General Chemistry
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy

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10.1038/s41467-017-01891-9

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AU - Russell-Pavier, Freddie

AU - Yacoot, Andrew

AU - Picco, Loren

AU - Payton, Oliver

AU - Toor, Amir

AU - Chesney, Alden

AU - Gimzewski, James K.

AU - Mishra, Bud

AU - Reed, Jason

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DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle

Abstract

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