Frog kidney cells in tissue culture exhibit a lag period of about 48 hr before the onset of DNA synthesis. During this time the number of cells incorporating nucleoside into rapidly labeled RNA decreases for 10-12 hr, then increases until 48 hr. Coincident with the start of DNA synthesis there is a sharp drop in the synthesis of rapidly labeled RNA. The generation cycle of frog kidney cells was determined: G1 + telophase = 16 hr, S = 22.3 hr, G2 + prophase = 7.7 hr, T = 46 hr.
ASJC Scopus subject areas
- Cell Biology