DPY-27: A chromosome condensation protein homolog that regulates C. elegans dosage compensation through association with the X chromosome

Pao Tien Chuang; Donna G. Albertson; Barbara J. Meyer

doi:10.1016/0092-8674(94)90255-0

DPY-27: A chromosome condensation protein homolog that regulates C. elegans dosage compensation through association with the X chromosome

Pao Tien Chuang, Donna G. Albertson, Barbara J. Meyer

Oral and Maxillofacial Surgery

Research output: Contribution to journal › Article › peer-review

Abstract

dpy-27 is an essential dosage compensation gene that acts to reduce expression of both hermaphrodite X chromosomes. The DPY-27 protein becomes specifically localized to the X chromosomes of wild-type XX embryos, but remains diffusely distributed throughout the nuclei of male (XO) embryos. In xol-1 mutant XO embryos that activate the XX mode of dosage compensation and die from inappropriately low X chromosome transcript levels, DPY-27 becomes localized to X. Therefore, sex specificity of the dosage compensation process is regulated at the step of DPY-27 X chromosome localization. DPY-27 exhibits striking similarity to proteins required for assembly and structural maintenance of Xenopus chromosomes in vitro and for segregation of yeast chromosomes in vivo. These findings suggest a link between global regulation of gene expression and higher order chromosome structure. We propose that DPY-27 implements dosage compensation by condensing the chromatin structure of X in a manner that causes general reduction of X chromosome expression.

Original language	English (US)
Pages (from-to)	459-474
Number of pages	16
Journal	Cell
Volume	79
Issue number	3
DOIs	https://doi.org/10.1016/0092-8674(94)90255-0
State	Published - Nov 4 1994

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/0092-8674(94)90255-0

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title = "DPY-27: A chromosome condensation protein homolog that regulates C. elegans dosage compensation through association with the X chromosome",

abstract = "dpy-27 is an essential dosage compensation gene that acts to reduce expression of both hermaphrodite X chromosomes. The DPY-27 protein becomes specifically localized to the X chromosomes of wild-type XX embryos, but remains diffusely distributed throughout the nuclei of male (XO) embryos. In xol-1 mutant XO embryos that activate the XX mode of dosage compensation and die from inappropriately low X chromosome transcript levels, DPY-27 becomes localized to X. Therefore, sex specificity of the dosage compensation process is regulated at the step of DPY-27 X chromosome localization. DPY-27 exhibits striking similarity to proteins required for assembly and structural maintenance of Xenopus chromosomes in vitro and for segregation of yeast chromosomes in vivo. These findings suggest a link between global regulation of gene expression and higher order chromosome structure. We propose that DPY-27 implements dosage compensation by condensing the chromatin structure of X in a manner that causes general reduction of X chromosome expression.",

author = "Chuang, {Pao Tien} and Albertson, {Donna G.} and Meyer, {Barbara J.}",

note = "Funding Information: Correspondence should be addressed to B. J. M. We are indebted to David Hsu for the initial RFLP mapping of dpy-27. We thank Tatsuya Hirano, Tim Mitchison, Alexender Strunnikov, and Douglous Koshland for communicating sequence information prior to publication. We thank Jau-Nian Chen for assistance in cDNA sequencing, Steve Hol-brook and Dek Woolfson for making the secondary structure predictions, Jenny Kopczynski for providing the him-8 yls2(xoC7::lecZ) strains, and Lori Sussel and Gene Cutler for help in image processing. We thank Tom Cline, Mike Botchan, and members of the Meyer lab, especially Lori Sussel and Nick Rhind, for advice, numerous helpful discussions, and critical comments on the manuscript. This work was supported by National Institutes of Health grant GM30702 and American Cancer Society grant DB-5 awarded to B. J. M.",

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AU - Meyer, Barbara J.

N1 - Funding Information: Correspondence should be addressed to B. J. M. We are indebted to David Hsu for the initial RFLP mapping of dpy-27. We thank Tatsuya Hirano, Tim Mitchison, Alexender Strunnikov, and Douglous Koshland for communicating sequence information prior to publication. We thank Jau-Nian Chen for assistance in cDNA sequencing, Steve Hol-brook and Dek Woolfson for making the secondary structure predictions, Jenny Kopczynski for providing the him-8 yls2(xoC7::lecZ) strains, and Lori Sussel and Gene Cutler for help in image processing. We thank Tom Cline, Mike Botchan, and members of the Meyer lab, especially Lori Sussel and Nick Rhind, for advice, numerous helpful discussions, and critical comments on the manuscript. This work was supported by National Institutes of Health grant GM30702 and American Cancer Society grant DB-5 awarded to B. J. M.

PY - 1994/11/4

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N2 - dpy-27 is an essential dosage compensation gene that acts to reduce expression of both hermaphrodite X chromosomes. The DPY-27 protein becomes specifically localized to the X chromosomes of wild-type XX embryos, but remains diffusely distributed throughout the nuclei of male (XO) embryos. In xol-1 mutant XO embryos that activate the XX mode of dosage compensation and die from inappropriately low X chromosome transcript levels, DPY-27 becomes localized to X. Therefore, sex specificity of the dosage compensation process is regulated at the step of DPY-27 X chromosome localization. DPY-27 exhibits striking similarity to proteins required for assembly and structural maintenance of Xenopus chromosomes in vitro and for segregation of yeast chromosomes in vivo. These findings suggest a link between global regulation of gene expression and higher order chromosome structure. We propose that DPY-27 implements dosage compensation by condensing the chromatin structure of X in a manner that causes general reduction of X chromosome expression.

AB - dpy-27 is an essential dosage compensation gene that acts to reduce expression of both hermaphrodite X chromosomes. The DPY-27 protein becomes specifically localized to the X chromosomes of wild-type XX embryos, but remains diffusely distributed throughout the nuclei of male (XO) embryos. In xol-1 mutant XO embryos that activate the XX mode of dosage compensation and die from inappropriately low X chromosome transcript levels, DPY-27 becomes localized to X. Therefore, sex specificity of the dosage compensation process is regulated at the step of DPY-27 X chromosome localization. DPY-27 exhibits striking similarity to proteins required for assembly and structural maintenance of Xenopus chromosomes in vitro and for segregation of yeast chromosomes in vivo. These findings suggest a link between global regulation of gene expression and higher order chromosome structure. We propose that DPY-27 implements dosage compensation by condensing the chromatin structure of X in a manner that causes general reduction of X chromosome expression.

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DPY-27: A chromosome condensation protein homolog that regulates C. elegans dosage compensation through association with the X chromosome

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