@article{b7fa768e789c43939c35f80756fb684a,
title = "Drug response and genetic characterization of plasmodium falciparum clones recently isolated from a sudanese village",
abstract = "We have isolated 20 clones of Plasmodium falciparum from isolates from patients attending a village clinic in Sudan during 10 d in October–November 1989. The clones were genetically diverse, having highly variable molecular karyotypes and a wide range of drug responses. Chloroquine-sensitive (50% inhibitory concentration [IC50] in the 4–15 nm range) and chloroquine-resistant clones (IC50 in the 40–95 nm range) co-existed in the population, but no obvious amplification of the P-glycoprotein homologue gene, Pgh1 (previously known as the multi-drug resistance gene, mdr1) marked the chloroquine-resistant clones. Chloroquine resistance was reversible by verapamil in these clones, although they varied in their susceptibility to verapamil alone. These observations indicate that the biochemical characteristics of the Sudanese chloroquine-resistant P. falciparum are similar to those reported from south-east Asian and Latin American isolates, which is consistent with there being a similar molecular basis for this phenomenon.",
author = "Bayoumi, {Riad A L} and Creasey, {Alison M.} and Babiker, {Hamza A.} and Carlton, {Jane M R} and Sultan, {Ali A.} and Gwiria Satti and Sohal, {Awinder K.} and David Walliker and Jensen, {James B.} and Arnot, {David E.}",
note = "Funding Information: Acknowledgements Wet hankD rs Thor Theandera nd Lars Hviid of Copenhagen State University Hospital for their help and support in the SudanM alaria Project. We also thank the Malaria Administration of the SudaneseM inistry of Health for their advicea nd support for this nroiect.D r Iana McBride kindlv suooliedu s with her panelo f P. ~alcipatu~a llele-specificm on&clo~ antibodies, and Drs John Hyde and Alan Cowmank indly suppliedp robes for theD HFR and Pghl genes.T his work wass upportedb y the Wellcomet rust, the UK Medical ResearchC ouncil, US NIH grant Al-16312 and WHO (TDR) Institution Strengthening Grant 890297H. amza Babiker and Ah Sultana re supportedb y British Council StudentshipsR, iad Bayoumi and David Arnot held a WellcomeT ravelling Fellowship and Senior Biomedical ResearchF ellowship, respectively.T his work would not have been possiblew ithout the support and goodwill of the staff of GedarefH ospitala nd thev illagerso f Asar, Sudan.",
year = "1993",
month = jul,
doi = "10.1016/0035-9203(93)90034-N",
language = "English (US)",
volume = "87",
pages = "454--458",
journal = "Transactions of the Royal Society of Tropical Medicine and Hygiene",
issn = "0035-9203",
publisher = "Oxford University Press",
number = "4",
}