TY - JOUR
T1 - Dynamic FoxG1 Expression Coordinates the Integration of Multipolar Pyramidal Neuron Precursors into the Cortical Plate
AU - Miyoshi, Goichi
AU - Fishell, Gord
N1 - Funding Information:
Research in Fishell lab is supported by the National Institutes of Health (grants RO1NS039007 and RO1MH071679) and the Simons Foundation and New York State through its NYSTEM initiative. G.M. is supported by a grant from the National Alliance for Research on Schizophrenia and Depression. We thank the following doctors for kindly sharing their reagents: David Anderson (Neurog2-CreER driver), Yoshiki Sasai (FoxG1 antibodies), Sally Temple (FoxG1 antibodies), Jean Hebert (Targeting arms for the FoxG1 locus), Toshifumi Morimura (mDab1 DNA construct), Eseng Lai (FoxG1-LacZ knockin mutant), Pierre Mattar and Carol Schuurmans (NeuroD1 promoter pGL3 construct), Kyonsoo Hong (Rat Dcc DNA construct), Takahiko Matsuda and Connie Cepko (CAGEN vector), Rudiger Klein (Flrt1-3 DNA constructs), and Nobuhiko Yamamoto (Netrin4 DNA construct). We also wish to thank Dr. Alex Joyner for helpful inputs constructing the conditional FoxG1 loss-of-function allele and Dr. Frada Berenshteyn for her generous help in gene targeting and ES cell selection. We thank Lihong Yin for her technical help. We greatly appreciate Dr. Vitor Sousa for his collaborative effort in generating the RCE EGFP reporter lines. We especially wish to thank Dr. Rob Machold for his intellectual inputs in the interpretation of our data and for the generous time he devoted in discussions and assembly of this manuscript. We are also greatly appreciative of the efforts Drs. Theofanis Karayannis, Xavier Jaglin and Allison Roberta made in critically reading this manuscript. Finally, we are extremely appreciative to all of the Fishell lab members for the support and suggestions throughout this project.
PY - 2012/6/21
Y1 - 2012/6/21
N2 - Pyramidal cells of the cerebral cortex are born in the ventricular zone and migrate through the intermediate zone to enter into the cortical plate. In the intermediate zone, these migrating precursors move tangentially and initiate the extension of their axons by transiently adopting a characteristic multipolar morphology. We observe that expression of the forkhead transcription factor . FoxG1 is dynamically regulated during this transitional period. By utilizing conditional genetic strategies, we show that the downregulation of . FoxG1 at the beginning of the multipolar cell phase induces . Unc5D expression, the timing of which ultimately determines the laminar identity of pyramidal neurons. In addition, we demonstrate that the re-expression of . FoxG1 is required for cells to transit out of the multipolar cell phase and to enter into the cortical plate. Thus, the dynamic expression of . FoxG1 during migration within the intermediate zone is essential for the proper assembly of the cerebral cortex.
AB - Pyramidal cells of the cerebral cortex are born in the ventricular zone and migrate through the intermediate zone to enter into the cortical plate. In the intermediate zone, these migrating precursors move tangentially and initiate the extension of their axons by transiently adopting a characteristic multipolar morphology. We observe that expression of the forkhead transcription factor . FoxG1 is dynamically regulated during this transitional period. By utilizing conditional genetic strategies, we show that the downregulation of . FoxG1 at the beginning of the multipolar cell phase induces . Unc5D expression, the timing of which ultimately determines the laminar identity of pyramidal neurons. In addition, we demonstrate that the re-expression of . FoxG1 is required for cells to transit out of the multipolar cell phase and to enter into the cortical plate. Thus, the dynamic expression of . FoxG1 during migration within the intermediate zone is essential for the proper assembly of the cerebral cortex.
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U2 - 10.1016/j.neuron.2012.04.025
DO - 10.1016/j.neuron.2012.04.025
M3 - Article
C2 - 22726835
AN - SCOPUS:84862689222
SN - 0896-6273
VL - 74
SP - 1045
EP - 1058
JO - Neuron
JF - Neuron
IS - 6
ER -