TY - JOUR
T1 - Dynamic loading stimulates mandibular condyle remodeling
AU - Teixeira, Cristina C.
AU - Abdullah, Fanar
AU - Alikhani, Mona
AU - Alansari, Sarah
AU - Sangsuwon, Chinapa
AU - Oliveira, Serafim
AU - Nervina, Jeanne M.
AU - Alikhani, Mani
N1 - Funding Information:
Funding: All work described in this manuscript was supported by funds from CTOR, Consortium for Translational Orthodontics Research, Hoboken, New Jersey.
Publisher Copyright:
© 2022 World Federation of Orthodontists
PY - 2022/10
Y1 - 2022/10
N2 - Background: We and others have reported that low-magnitude high-frequency dynamic loading has an osteogenic effect on alveolar bone. Since chondrocytes and osteoblasts originate from the same progenitor cells, we reasoned that dynamic loading may stimulate a similar response in chondrocytes. A stimulating effect could be beneficial for patients with damaged condylar cartilage or mandibular deficiency. Methods: Studies were conducted on growing Sprague-Dawley rats divided into three groups: control, static load, and dynamic load. The dynamic load group received a dynamic load on the lower right molars 5 minutes per day with a 0.3 g acceleration and peak strain of 30 με registered by accelerometer and strain gauge. The static load group received an equivalent magnitude of static force (30 με). The control group did not receive any treatment. Samples were collected at days 0, 28, and 56 for reverse transcriptase polymerase chain reaction analysis, microcomputed tomography, and histology and fluorescent microscopy analysis. Results: Our experiments showed that dynamic loading had a striking effect on condylar cartilage, increasing the proliferation and differentiation of mesenchymal cells into chondrocytes, and promoting chondrocyte maturation. This effect was accompanied by increased endochondral bone formation resulting in lengthening of the condylar process. Conclusions: Low-magnitude, high-frequency dynamic loading can have a positive effect on condylar cartilage and endochondral bone formation in vivo. This effect has the potential to be used as a treatment for regenerating condylar cartilage and to enhance the effect of orthopedic appliances on mandibular growth.
AB - Background: We and others have reported that low-magnitude high-frequency dynamic loading has an osteogenic effect on alveolar bone. Since chondrocytes and osteoblasts originate from the same progenitor cells, we reasoned that dynamic loading may stimulate a similar response in chondrocytes. A stimulating effect could be beneficial for patients with damaged condylar cartilage or mandibular deficiency. Methods: Studies were conducted on growing Sprague-Dawley rats divided into three groups: control, static load, and dynamic load. The dynamic load group received a dynamic load on the lower right molars 5 minutes per day with a 0.3 g acceleration and peak strain of 30 με registered by accelerometer and strain gauge. The static load group received an equivalent magnitude of static force (30 με). The control group did not receive any treatment. Samples were collected at days 0, 28, and 56 for reverse transcriptase polymerase chain reaction analysis, microcomputed tomography, and histology and fluorescent microscopy analysis. Results: Our experiments showed that dynamic loading had a striking effect on condylar cartilage, increasing the proliferation and differentiation of mesenchymal cells into chondrocytes, and promoting chondrocyte maturation. This effect was accompanied by increased endochondral bone formation resulting in lengthening of the condylar process. Conclusions: Low-magnitude, high-frequency dynamic loading can have a positive effect on condylar cartilage and endochondral bone formation in vivo. This effect has the potential to be used as a treatment for regenerating condylar cartilage and to enhance the effect of orthopedic appliances on mandibular growth.
KW - Cell proliferation
KW - Chondrogenic markers
KW - Condylar growth
KW - Dynamic loading
KW - Endochondral bone formation
KW - Osteogenic effect
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U2 - 10.1016/j.ejwf.2022.08.002
DO - 10.1016/j.ejwf.2022.08.002
M3 - Article
C2 - 36153283
AN - SCOPUS:85138480615
SN - 2212-4438
VL - 11
SP - 146
EP - 155
JO - Journal of the World Federation of Orthodontists
JF - Journal of the World Federation of Orthodontists
IS - 5
ER -