TY - JOUR
T1 - Dynamic profiling of the protein life cycle in response to pathogens
AU - Jovanovic, Marko
AU - Rooney, Michael S.
AU - Mertins, Philipp
AU - Przybylski, Dariusz
AU - Chevrier, Nicolas
AU - Satija, Rahul
AU - Rodriguez, Edwin H.
AU - Fields, Alexander P.
AU - Schwartz, Schraga
AU - Raychowdhury, Raktima
AU - Mumbach, Maxwell R.
AU - Eisenhaure, Thomas
AU - Rabani, Michal
AU - Gennert, Dave
AU - Lu, Diana
AU - Delorey, Toni
AU - Weissman, Jonathan S.
AU - Carr, Steven A.
AU - Hacohen, Nir
AU - Regev, Aviv
PY - 2015/3/6
Y1 - 2015/3/6
N2 - Protein expression is regulated by the production and degradation of messenger RNAs (mRNAs) and proteins, but their specific relationships remain unknown. We combine measurements of protein production and degradation and mRNA dynamics so as to build a quantitative genomic model of the differential regulation of gene expression in lipopolysaccharide-stimulated mouse dendritic cells. Changes in mRNA abundance play a dominant role in determining most dynamic fold changes in protein levels. Conversely, the preexisting proteome of proteins performing basic cellular functions is remodeled primarily through changes in protein production or degradation, accounting for more than half of the absolute change in protein molecules in the cell. Thus, the proteome is regulated by transcriptional induction for newly activated cellular functions and by protein life-cycle changes for remodeling of preexisting functions.
AB - Protein expression is regulated by the production and degradation of messenger RNAs (mRNAs) and proteins, but their specific relationships remain unknown. We combine measurements of protein production and degradation and mRNA dynamics so as to build a quantitative genomic model of the differential regulation of gene expression in lipopolysaccharide-stimulated mouse dendritic cells. Changes in mRNA abundance play a dominant role in determining most dynamic fold changes in protein levels. Conversely, the preexisting proteome of proteins performing basic cellular functions is remodeled primarily through changes in protein production or degradation, accounting for more than half of the absolute change in protein molecules in the cell. Thus, the proteome is regulated by transcriptional induction for newly activated cellular functions and by protein life-cycle changes for remodeling of preexisting functions.
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U2 - 10.1126/science.1259038
DO - 10.1126/science.1259038
M3 - Article
C2 - 25745177
AN - SCOPUS:84924347993
SN - 0036-8075
VL - 347
JO - Science
JF - Science
IS - 6226
M1 - 1259038
ER -