Abstract
An in vitro model was developed to characterize the effects of various biomaterial substrates on the early events of periodontal connective tissue attachment formation. Primary cultures of periodontal ligament and gingival connective tissue cells were cultured on uncoated (control) and coated (titanium- and hydroxyapatite-coated_ tissue culture plastic, and the level of cell proliferation, collagen, and noncollagen protein synthesis, alkaline phosphatase activity, and expression of a 42 kD cementum extracellular matrix protein were measured over 5, 7, and 9 days in culture. The results serve to validate the model.
Original language | English (US) |
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Pages (from-to) | 585-594 |
Number of pages | 10 |
Journal | Journal of Biomedical Materials Research |
Volume | 47 |
Issue number | 4 |
DOIs | |
State | Published - Dec 15 1997 |
Keywords
- Cementum
- Collagen synthesis
- Hydroxyapatite
- Periodontal connective tissues
- Titanium
ASJC Scopus subject areas
- Biomaterials
- Biomedical Engineering