TY - JOUR
T1 - Effect of 5-HT depletion of the hippocampus on neuronal transmission from perforant path through dentate gyrus
AU - Srebro, B.
AU - Azmitia, E. C.
AU - Winson, J.
N1 - Funding Information:
This work was supported by Grant BNS 79-06474 from the National Science Foundation to E.C.A. and by Research Career Development Award 5-KO2-MH 00232 from the National Institute of Mental Health, Grant BNS 79-09024 from the National Science Foundation and by a grant from the Harry F. Guggenheim Foundation to J.W.
PY - 1982/3/4
Y1 - 1982/3/4
N2 - Stimulation of the perforant pathway (pp) elicits a characteristics evoked action potential (EAP) in the granule cell layer of the dentate gyrus. The EAP was recorded in rats depleted of hippocampal serotonin (5-HT) by prior injection of p-chloroamphetamine (PCA) or 5,7-dihydroxytryptamine (5,7-DHT) as well as in untreated animals during two behavioral states, slow-wave sleep (SWS) and still-alert behavior (SAL). As reported previously13,14, in untreated rats the amplitude of the EAP response was significantly greater during SWS than SAL. Stimulation of the median raphe nucleus (MR) prior to stimulating the pp (prestimulation) augmented the EAP response, but only during SWS. In contrast, in animals injected with PCA or 5,7-DHT there was no difference of the amplitude of the EAP during SWS and SAL. However, the augmentation of the EAP during SWS produced by prestimulation of the median raphe was still present. It is concluded that 5-HT innervation of the dentate gyrus may be involved in the behavioral modulation of the EAP response. Modulation of the EAP following prestimulation of the MR appears to be effected by a non-serotonergic input to the dentate gyrus originating in, or coursing through, the median raphe.
AB - Stimulation of the perforant pathway (pp) elicits a characteristics evoked action potential (EAP) in the granule cell layer of the dentate gyrus. The EAP was recorded in rats depleted of hippocampal serotonin (5-HT) by prior injection of p-chloroamphetamine (PCA) or 5,7-dihydroxytryptamine (5,7-DHT) as well as in untreated animals during two behavioral states, slow-wave sleep (SWS) and still-alert behavior (SAL). As reported previously13,14, in untreated rats the amplitude of the EAP response was significantly greater during SWS than SAL. Stimulation of the median raphe nucleus (MR) prior to stimulating the pp (prestimulation) augmented the EAP response, but only during SWS. In contrast, in animals injected with PCA or 5,7-DHT there was no difference of the amplitude of the EAP during SWS and SAL. However, the augmentation of the EAP during SWS produced by prestimulation of the median raphe was still present. It is concluded that 5-HT innervation of the dentate gyrus may be involved in the behavioral modulation of the EAP response. Modulation of the EAP following prestimulation of the MR appears to be effected by a non-serotonergic input to the dentate gyrus originating in, or coursing through, the median raphe.
KW - dentate gyrus
KW - freely moving rats
KW - neuronal transmission
KW - serotonin
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U2 - 10.1016/0006-8993(82)90204-9
DO - 10.1016/0006-8993(82)90204-9
M3 - Article
C2 - 6329412
AN - SCOPUS:0020072393
VL - 235
SP - 142
EP - 147
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1
ER -