Effect of a novel type of propolis and its chemical fractions on glucosyltransferases and on growth and adherence of mutans streptococci

Simone Duarte, Hyun Koo, William Henry Bowen, Mitsue Fujimaki Hayacibara, Jaime Aparecido Cury, Masaharu Ikegaki, Pedro Luiz Rosalen

    Research output: Contribution to journalArticlepeer-review


    Flavonoids have been considered the main biologically active components in propolis. However, a new variety of flavonoid-free propolis was recently found and chemically classified as type 6. Because it showed activity against oral microorganisms, this study evaluated the effects of the crude ethanolic extract of this propolis and its chemical fractions on the activity of purified glucosyltransferases (GTFs) and on the growth and adherence of mutans streptococci. The inhibitory effect of propolis extracts on GTF activities was determined either in solution or adsorbed onto saliva-coated hydroxyapatite. Streptococcus mutans Ingbritt 1600, Streptococcus sobrinus 6715, and two clinical isolates of each species were used for antibacterial assays. Susceptibilities to the test extracts were analyzed using the agar diffusion method and by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC); the effect on bacterial adherence to a glass surface was also assessed. The activity of GTFs in solution was effectively inhibited by the ethanolic extract of propolis type 6 (EEP) (>80% inhibition at 0.5 mg/ml), hexane, and chloroform fractions (60-90% inhibition at 100 μg/ml); their inhibitory effects on surface enzymes were less pronounced. The EEP, hexane, and chloroform fractions also showed significant antibacterial activity. The data showed that propolis type 6 remarkably reduced GTF activity and inhibited mutans streptococci growth and adherence; these biological activities are associated with its nonpolar components.

    Original languageEnglish (US)
    Pages (from-to)527-531
    Number of pages5
    JournalBiological and Pharmaceutical Bulletin
    Issue number4
    StatePublished - Apr 2003


    • Adherence
    • Antimicrobial activity
    • Glucosyltransferase
    • Mutans streptococci
    • Propolis

    ASJC Scopus subject areas

    • Pharmacology
    • Pharmaceutical Science


    Dive into the research topics of 'Effect of a novel type of propolis and its chemical fractions on glucosyltransferases and on growth and adherence of mutans streptococci'. Together they form a unique fingerprint.

    Cite this