Effect of partially demineralized dentin beneath the hybrid layer on dentin-adhesive interface micromechanics

Rodolfo Bruniera Anchieta, Lucas Silveira Machado, Renato Herman Sundfeld, André Figueiredo Reis, Marcelo Giannini, Marco Antonio Luersen, Malvin Janal, Eduardo Passos Rocha, Paulo G. Coelho

Research output: Contribution to journalArticlepeer-review


Objective: To investigate the presence of non-infiltrated, partially demineralized dentin (PDD) beneath the hybrid layer for self-etch adhesive systems, and its effect on micromechanical behavior of dentin-adhesive interfaces (DAIs). This in-vitro laboratory and computer simulation study hypothesized that the presence of non-infiltrated PDD beneath the hybrid layer does not influence the mechanical behavior of the DAI of self-etch adhesive systems. Methods: Fifteen sound third molars were restored with composite resin using three adhesive systems: Scotchbond Multipurpose (SBMP), Clearfil SE Bond (CSEB) and Adper Promp L-Pop (APLP). The thickness and length of all DAIs were assessed using scanning electron microscopy, and used to generate three-dimensional finite element models. Elastic moduli of the hybrid layer, adhesive layer, intertubular dentin, peritubular dentin and resin tags were acquired using a nano-indenter. Finite element software was used to determine the maximum principal stress. Mixed models analysis of variance was used to verify statistical differences (. P<0.05). Results: Elastic moduli and morphology were found to differ between the adhesive systems, as well as the presence and extension of PDD. Significance: Both self-etch adhesive systems (APLP and CSEB) had PDD. The DAI stress levels were higher for the one-step self-etch adhesive system (APLP) compared with the etch-and-rinse adhesive system (SBMP) and the self-etch primer system (CSEB).

Original languageEnglish (US)
Pages (from-to)701-707
Number of pages7
JournalJournal of Biomechanics
Issue number4
StatePublished - Feb 26 2015


  • Adhesion
  • Adhesive systems
  • Dentin
  • Finite element
  • Hybrid layer
  • Mechanical properties
  • Nano-indentation

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Orthopedics and Sports Medicine
  • Rehabilitation


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