Effect of photodynamic antimicrobial chemotherapy on in vitro and in situ biofilms

A. H. Teixeira; E. S. Pereira; L. K.A. Rodrigues; D. Saxena; S. Duarte; I. C.J. Zanin

doi:10.1159/000341190

Effect of photodynamic antimicrobial chemotherapy on in vitro and in situ biofilms

A. H. Teixeira, E. S. Pereira, L. K.A. Rodrigues, D. Saxena, S. Duarte, I. C.J. Zanin

Molecular Pathobiology

Research output: Contribution to journal › Article › peer-review

Abstract

Aim: The effect of photodynamic antimicrobial chemotherapy (PACT) on oral biofilms was evaluated. Methods: Biofilms formed in vitro were treated with sensitizer (S) and/or light (L) in the test (S⁺L⁺) and control (S^-L^-, S⁺L^-, S ^-L⁺) groups. Additionally, 21 volunteers wore intraoral devices and the biofilms formed on each side of the device were referred to as S⁺L⁺ or S^-L^- groups. Results: Significant decreases in the viability of in vitro biofilms were observed after PACT. PACT was ineffective in inhibiting multi-species biofilms formed in situ. Conclusion: PACT was effective on in vitro biofilms and was ineffective on in situ biofilms.

Original language	English (US)
Pages (from-to)	549-554
Number of pages	6
Journal	Caries research
Volume	46
Issue number	6
DOIs	https://doi.org/10.1159/000341190
State	Published - Dec 2012

Keywords

Biofilms
In situ study
Photodynamic antimicrobial chemotherapy
Streptococcus mutans

ASJC Scopus subject areas

General Dentistry

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10.1159/000341190

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title = "Effect of photodynamic antimicrobial chemotherapy on in vitro and in situ biofilms",

abstract = "Aim: The effect of photodynamic antimicrobial chemotherapy (PACT) on oral biofilms was evaluated. Methods: Biofilms formed in vitro were treated with sensitizer (S) and/or light (L) in the test (S+L+) and control (S-L-, S+L-, S -L+) groups. Additionally, 21 volunteers wore intraoral devices and the biofilms formed on each side of the device were referred to as S+L+ or S-L- groups. Results: Significant decreases in the viability of in vitro biofilms were observed after PACT. PACT was ineffective in inhibiting multi-species biofilms formed in situ. Conclusion: PACT was effective on in vitro biofilms and was ineffective on in situ biofilms.",

keywords = "Biofilms, In situ study, Photodynamic antimicrobial chemotherapy, Streptococcus mutans",

author = "Teixeira, {A. H.} and Pereira, {E. S.} and Rodrigues, {L. K.A.} and D. Saxena and S. Duarte and Zanin, {I. C.J.}",

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language = "English (US)",

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TY - JOUR

T1 - Effect of photodynamic antimicrobial chemotherapy on in vitro and in situ biofilms

AU - Teixeira, A. H.

AU - Pereira, E. S.

AU - Rodrigues, L. K.A.

AU - Saxena, D.

AU - Duarte, S.

AU - Zanin, I. C.J.

PY - 2012/12

Y1 - 2012/12

N2 - Aim: The effect of photodynamic antimicrobial chemotherapy (PACT) on oral biofilms was evaluated. Methods: Biofilms formed in vitro were treated with sensitizer (S) and/or light (L) in the test (S+L+) and control (S-L-, S+L-, S -L+) groups. Additionally, 21 volunteers wore intraoral devices and the biofilms formed on each side of the device were referred to as S+L+ or S-L- groups. Results: Significant decreases in the viability of in vitro biofilms were observed after PACT. PACT was ineffective in inhibiting multi-species biofilms formed in situ. Conclusion: PACT was effective on in vitro biofilms and was ineffective on in situ biofilms.

AB - Aim: The effect of photodynamic antimicrobial chemotherapy (PACT) on oral biofilms was evaluated. Methods: Biofilms formed in vitro were treated with sensitizer (S) and/or light (L) in the test (S+L+) and control (S-L-, S+L-, S -L+) groups. Additionally, 21 volunteers wore intraoral devices and the biofilms formed on each side of the device were referred to as S+L+ or S-L- groups. Results: Significant decreases in the viability of in vitro biofilms were observed after PACT. PACT was ineffective in inhibiting multi-species biofilms formed in situ. Conclusion: PACT was effective on in vitro biofilms and was ineffective on in situ biofilms.

KW - Biofilms

KW - In situ study

KW - Photodynamic antimicrobial chemotherapy

KW - Streptococcus mutans

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Effect of photodynamic antimicrobial chemotherapy on in vitro and in situ biofilms

Abstract

Keywords

ASJC Scopus subject areas

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