The spatial non‐uniformity of a light beam must be taken into account in interpreting phenomena which depend on the light intensity in a non‐linear manner. Omission of this effect may lead to incorrect values of parameters obtained from a fitting of theoretical curves, which describe the dependence of a given phenomenon on light intensity, to the experimental data. The effect of spatial non‐uniformity of excitation light beams is illustrated utilizing non‐linear intensity‐dependent fluorescence phenomena in photosvnthetic svstems and a Gaussian light beam profile.
|Number of pages
|Photochemistry and photobiology
|Published - Apr 1983
ASJC Scopus subject areas
- Physical and Theoretical Chemistry