Effect of supplemental acid-etching on the early stages of osseointegration: A preclinical model

Ernesto B. Benalcázar Jalkh, Marcelo Parra, Andrea Torroni, Vasudev Vivekanand Nayak, Nick Tovar, Arthur Castellano, Rafael M. Badalov, Estevam A. Bonfante, Paulo G. Coelho, Lukasz Witek

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: To evaluate the effect of two surface modifications on early osseointegration parameters of conical implants in a translational pre-clinical model. Materials and methods: Conical implants with progressive trapezoidal threads and healing chambers were evaluated consisting of two different surface conditions: 1) Implacil surface (IMP Sur), and 2) Implacil surface + Supplemental Acid-etching (IMP Sur + AE). Surface characterization comprised of the evaluation of roughness parameters (Sa, Sq and Sdr), surface energy and contact angle. Subsequently, implants were installed in the ilium crest of nine female sheep (weighing ~65 kg). Torque out, histological and histomorphometric analyses were conducted after 3 and 6 weeks in-vivo. The percentage of bone to implant contact (%BIC) and bone area fraction occupancy within implant threads (%BAFO) were quantified, and the results were analyzed using a general linear mixed model analysis as function of surface treatment and time in-vivo. Results: Supplemental acid etching significantly increased Sa and Sq roughness parameters without compromising the surface energy or contact angle, and no significant differences with respect to Sdr. Torque-out testing yielded significantly higher values for IMP Sur + AE in comparison to the IMP Sur at 3- (62.78 ± 15 and 33.49 ± 15 N.cm, respectively) and 6-weeks (60.74 ± 15 and 39.80 ± 15 N.cm, respectively). Histological analyses depicted similar osseointegration features for both surfaces, where an intramembranous-type healing pattern was observed. At histomorphometric analyses, IMP Sur + AE implants yielded higher values of BIC in comparison to IMP Sur at 3- (40.48 ± 38 and 27.98 ± 38%, respectively) and 6-weeks (45.86 ± 38 and 34.46 ± 38%, respectively). Both groups exhibited a significant increase in %BAFO from 3 (~35%) to 6 weeks (~44%), with no significant differences between surface treatments. Conclusion: Supplemental acid-etching and its interplay with implant thread design, positively influenced the BIC and torque-out resistance at early stages of osseointegration.

Original languageEnglish (US)
Article number104682
JournalJournal of the Mechanical Behavior of Biomedical Materials
Volume122
DOIs
StatePublished - Oct 2021

Keywords

  • Dental implants
  • Design
  • Osseointegration
  • Surface treatment

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Mechanics of Materials

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